抗流感病毒卵黄免疫液抗病毒作用的研究

目的研制一种预防流感效果显著的免疫制剂。方法用流感病毒 (FM1株 )免疫母鸡 ,制备抗流感病毒 (FM1株 )卵黄免疫液。经动物实验 ,观察该免疫液抗流感病毒的效果。结果当对照组的小鼠开始病死时 ,生理盐水对照组、卵黄免疫液对照组与紧急预防组小鼠每日每只平均饮水量分别为 (3.0 6± 0 .86 )、(2 .93± 1.47)和(3.99± 0 .2 1)ml(P <0 .0 5 ) ,体重分别为 (15 .85± 2 .70 )、(14.5 8± 1.92 )和 (18.2 7± 1.71)g(P <0 .0 5 ) ;病死率分别为 5 3.84%、6 9.2 3%和 3.84% (P <0 .0 1) ;存活小鼠血清 (1∶10稀释 )抗体阳性率分别为 10 0 %、10 0 %和 0 (P <0 .0 1)。结论抗流感病毒卵黄免疫液抗病毒作用强 ,预防小鼠流感效果显著。

Study on anti-viruseffects of egg yolk immune solution of anti-influenza virus

Purpose　The aim is to find out an effective preventing influenza immune preparation. Methods　Egg yolk immune solution of anti-influenza virus(FM1 stem) was prepared from egg of the hens that had been successfully immunized with influenza virus (FM1 stem). Its effects of anti-virus were observed through animal experiment.Results　When the mice of control group began to die the average daily drink quantity of the mice of normal saline control group, egg yolk immune solution control group, immediate preventive group was (3.06±0.86), (2.93±1.47) and (3.99±0.21)ml(P＜0.05)respectively. The average body weights of the mice of these three groups were (15.85±2.70),(14.58±1.92) and (18.27±1.71)g(P＜0.05)respectively. Their mortality was separately 53.84%,69.23% and 3.84%(P＜0.01). The antibody positive rate of survived mice′s serum (1∶10 diluted) was separately 100%, 100% and 0%(P＜0.01). Conclusion　The anti-virus effects of egg yolk immune solution of anti-influenza virus was powerful. The result of preventing mice′s influenza was remarkable.