| 多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达 |
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| 引用本文： | 姜振宇,许颖,荆蕾,姚程,马宁.多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达[J].吉林大学学报(医学版),2010,36(2):312-315. |
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| 作者姓名： | 姜振宇 许颖 荆蕾 姚程 马宁 |
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| 作者单位： | 吉林大学第一医院风湿科,吉林,长春,130021;吉林大学第一医院,肾病内科,吉林,长春,130021;吉林大学第一医院血液肿瘤科,吉林,长春,130021 |
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| 基金项目： | 吉林省科技厅科研基金资助课题(200705140) |
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| 摘 要： | 目的:研究多药耐药相关蛋白-2（MRP-2）与白血病细胞耐药的关系，并探讨其在白血病多药耐药中的意义。方法：应用RT-PCR方法检测白血病细胞株K562与耐药细胞株K562/A02中MRP-2 mRNA的差异表达，然后将MRP-2反义及正义寡核苷酸片段分别用脂质体转染至耐药细胞株K562/A02，采用MTT法、流式细胞术及RT-PCR法检测转染后细胞内阿霉素的荧光强度、耐药指数及MRP-2 mRNA的表达情况。结果：耐药细胞株K562/A02中MRP-2 mRNA表达水平明显高于白血病细胞株K562（P<0.05）。MRP-2反义寡核苷酸片段转染后耐药细胞株K562/A02细胞内的阿霉素浓度升高，耐药指数较未转染细胞明显降低（P<0.05）；转染后耐药细胞株K562/A02细胞MRP-2 mRNA表达水平较未转染细胞下降了67.5%，两者比较差异具有显著性（P<0.05）。结论：MRP-2的高表达导致白血病耐药细胞内化疗药物浓度降低，可能是其导致白血病多药耐药的机制之一。
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| 关 键 词： | 白血病 多药耐药 多药耐药相关蛋白-2 |
| 收稿时间： | 2009-10-09 |
Expression of multi-drug resistance relative protein-2 in leukemia multi-drug resistance cell line of K562/A02 |
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| JIANG Zhen-yu,XU Ying,JING Lei,YAO Cheng,MA Ning.Expression of multi-drug resistance relative protein-2 in leukemia multi-drug resistance cell line of K562/A02[J].Journal of Jilin University: Med Ed,2010,36(2):312-315. |
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| Authors: | JIANG Zhen-yu XU Ying JING Lei YAO Cheng MA Ning |
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| Institution: | 1. Department of Rheumatology,First Hospital,Jilin University,Changchun 130021,China;2. Department of Nephrology,First Hospital,Jilin University,Changchun 130021,China;3. Department of Hematology and Oncology,First Hospital,Jilin University,Changchun 130021,China |
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| Abstract: | Objective To investigate the relationship between multi-drug resistance relative protein-2(MRP-2) and leukemia multi-drug resistance,and study its effect on leukemia multi-drug resistance.Methods The expressions of MRP-2 in leukemia cell line K562 and leukemia multi-drug resistance cell line K562/A02 were detected by PT-PCR.Anti-and oligonucleotide of MRP-2 were transfected by lipidosome into K562/A02,intra-cellular doxorubicin(ADM) fluorescence intensity,resistance index,and the expression of MRP-2 mRNA we... |
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| Keywords: | leukemia multi-drug resistance multi-drug resistance relative protein-2 |
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