一平苏治疗自发性高血压大鼠局灶性脑缺血的实验研究

目的研究一平苏对自发性高血压大鼠局灶性脑缺血的神经保护作用。方法线栓法制备自发性高血压大鼠局灶性脑缺血模型，一平苏治疗组造模前先治疗4周，进行行为观察后取脑作病理切片，用TTC染色，HE染色，尼氏染色，免疫组化的方法，通过图象分析测定脑梗塞面积，缺血区死亡神经细胞数目，尼氏染色阳性细胞以及HSP70与NF-kBP65免疫反应阳性细胞的光密度，分析一平苏的治疗作用及可能机制。结果一平苏使梗塞面积缩小，行为计分降低，死亡神经细胞数目减少，尼氏染色光密度增高，NF-kBP65阳性细胞光密度降低，对HSP70的表达无影响。结论一平苏能减轻自发性高血压大鼠局灶性脑缺血的梗塞程度，促进大鼠神经功能恢复，减少神经细胞坏死，保护神经细胞的功能，抑制NF-kB表达，一平苏有抑制炎症因子的作用，可能是其脑保护作用机制之一。

Effect of Cilazapril on Focal Cerebral Ischemia of Sponteaneous Hyperten sive Rats

Objective To investigate the protective effects of Cilazapri l on focal cerebral ischemia of spontaneous hypertensive rats.Method Focal cerebral ischemia of spontaneous hypertensive rats wer e made by occlusion of the middle cerebral artery(MCA). Cilazapril treated group was taken cilazapril 4 weeks before model was made. TTC staining,HE staining,Nis sl's staining,immunohistochemistry, and image analysis were taken to estimate th e area of ischemia,number of dead neural cells,optical density of positive cell of Nissl's staining,HSP70 and NF-k BP65 immunohistochemistry reaction in ischem ia area.Result Cilazapril could reduce the area of ischemia and behavior sc ores,decrease the number of died neural ce lls,increase the optical density of positive cell of Nissl's staining,reduce th e optical density of NF-k BP65. No effect on HSP70 immunohistochemistry reactio n was found.Conclusion Cilazapril could decrease infarction of focal ischemia o f spontaneous hypertensive rats, promote the functional restoration of rats,decr ease nerve cell death,inhibit the expression of NF-k B. Cilazapril inhibited in flammatory reaction and that could be one of its protective mechanisms.