聚酯酰胺静电纺纳米纤维膜的制备及其生物相容性

采用静电纺技术制备聚酯酰胺纳米纤维膜,用扫描电镜观察纳米纤维的表面形貌,发现随着纺丝液质量浓度的增加,纤维的直径由10%时的180 nm递增到20%时的350 nm;用红外光谱表征聚酯酰胺的化学结构,结果表明化学结构在纺丝前后没有发生明显变化;X射线衍射法测试表明聚酯酰胺在经过溶剂处理和纺丝后,结晶度下降;差示扫描量热法表征了热力学特性,发现聚酯酰胺经过溶剂处理和纺丝后,结晶度下降;力学性能测试表明平均厚度为(0.50±0.05)mm的聚酯酰胺纳米纤维膜的平均断裂强度和平均断裂伸长率分别达到(1.00±0.18)MPa和(18.20±2.86)%;MTT结果表明内皮细胞在聚酯酰胺纳米纤维膜上增殖活跃,内皮细胞在纤维表面黏附并显示出良好的生长形态.

Preparation and biocompatibility of poly ester-amide electrospinning nanofiber membrane

The poly ester-amide (PEA) nanofiber membrane was prepared by electrospinning and the surface topography of nanofiber membrane was observed by scanning electron microscope (SEM). It was found that with the increasing of spinning fluid concentration from 10% to 20%, the diameter of prepared fibers was increased from 180 nm to 305 nm. The chemical structure of PEA was detected by infrared spectrum, which showed that electrospinning had no significant effect on PEA. X-ray diffraction spectrogram and differential scanning calorimetry revealed that the crystallinity of PEA was decreased after electrospinning. Mechanical property test demonstrated that the mean breaking strength and elongation at break of PEA nanofiber membrane with (0.50±0.05) mm thickness were (1.00±0.18) MPa, and (18.20±2.86)%, respectively. MTT results showed that the endothelial cells proliferated actively on the PEA nanofiber membrane, and presented good adhesive and growth status.