紫外线诱变产黄青霉菌原生质体选育高产菌种

在菌种诱变筛选过程中，往往由于细胞壁的存在而影响其对外界理化因子的敏感性，因而我们采用细胞脱壁后再进行紫外线诱变处理的方法，选育青霉素高产优质菌株。分离纯化得到276#菌株，摇瓶考查青霉素效价和总产量分别提高了8．2％和6．4％，对氧的需求也有所降低，达到了预期效果。

Breeding the high-productive penicillin strain through mutate protoplast with ultrauiolet

During the selecting of fungus'mutation process,frequently sensitivity to the physics and chemistry divisor in the outside world was effect by the cell wall.As a result we adopt mutate protoplast with the ultraviolet ray after leave the cell wall to breeding the high-productive penicillin producing strain.Isolated and extracted product strain 276~#,the result of flask-shaking test showed that the penicillin fermentation and total yield increase 8.2%and 6.4%.The demand to oxygen also cuts down somewhat.Attaining anticipates effect.