外源性血管内皮生长因子对大鼠脊髓神经元的神经营养作用

目的 观察血管内皮生长因子(VEGF)对大鼠脊髓神经元的神经营养作用.方法 体外培养SD大鼠脊髓神经元细胞,加入不同浓度的VEGF164(10、25、50、75、100μg/L),通过神经元细胞计数、噻唑蓝(MTT)比色法检测神经元细胞活性、免疫缉织化学方法观察大鼠脊髓神经元VEGF以及其受体Flk-1/Fh-1表达情况,检测并测量微管相关蛋白(MAP)-2标记的神经轴突生长长度,用50 mg/L BrdU标记神经元前体细胞,研究VEGF164对体养的大鼠脊髓神经元前体细胞的增殖性的影响.结果 当培养液终质量浓度达到25μg/L时,受5'-溴脱氧尿嘧啶(BrdU)标记的神经元前体细胞阳性细胞数由36/1000增加到62/1000,神经元前体细胞轴突生长平均长度由0.021mm增加到0.037mm.SUl498可抑制VEGF的这一作用.结论 VEGFl164可能通过VEGFR2/flk-1受体途径介导,对大鼠脊髓神经元具有神经营养作用.

Neurotrophlc effect of vascular endothelial growth factor on rat spinal cord neuron in vitro

Objective To investigate direct neurotrophic effects of vascular endothelial growth factor (VEGF) on SD rat spinal cord neurons. Methods We employed primary cultures of embryonic rat spinal cord neurons,then administrated different concentration VEGF164 (10,25,50,75,100 μg/L) in culture medium, Using BrdU labeling (50 mg/L)as an index of neuronal precursors proliferation, the light microscope, MTT assay and immunochemistry were employed to measure and observe the change of rat spinal cord neuron in cell morphology, axonal growth, proliferation and expression of VEGF receptors. Results When VEGF concentrations in culture medium reached 25 μg/L, positive cells labeling with Brdu increased from 36/1000 to 62/1000,neurite length significantly increased from 0.021 mm to 0.037 mm. VEGFR2/FLK-1 inhibitor SU1498 can reverse these effects. Conclusion Vascular endothelial growth factor (VEGF) might stimulates Neurogenesis in Vitro Through VEGFR2/Flk-1 Receptors.