肺癌组织P-糖蛋白表达与原位细胞凋亡、增殖、凋亡相关基因关系及其临床意义

目的探讨肺癌组织P-糖蛋白(P-glycoprotein,P-gp)表达与细胞凋亡、增殖及凋亡相关基因的关系及其预后意义.方法应用末端脱氧核糖核酸转移酶法(TUNEL)检测凋亡细胞,免疫组织化学检测耐药及凋亡相关基因蛋白表达.结果非小细胞肺癌(NSCLC)P-糖蛋白阳性率22.2%,小细胞肺癌(SCLC)阳性率11.5%.凋亡指数(Apoptosisindex,AI)和增殖指数(PCNA-labelingindex,PCNA-LI)呈高度负相关(r=-0.7358,P<0.01).P-gp阳性者,AI和PCNA-LI高于P-gp阴性者,PCNA-LI差异有显著性(P<0.01).P-gp与bcl-2表达相关(r=0.5637,P<0.05),与P21WAF1/CIPI无关(r=0.2405,P>0.05).多因素Cox回归分析显示,P-gp及AI有预后意义.结论P-gp与细胞凋亡、增殖、凋亡相关基因相关,且具预后意义.

Expression and clinical significance of P-glycoprotein in lung cancer and itscorrelations with apoptosis,proliferation and apoptosis-related genes

Objective To investigate expression and prognostic significance of P-glycoprotein and its relations to apoptosis,proliferation and apoptosis-related genes in patients with lung cancer.Methods Terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) was used to evaluate apoptosis cells.The expression of bcl-2,P-gp,proliferating-cell nuclear antigen(PCNA)was measured by immunohistochemistry.Results P-gp was detected in 8 of 36 NSCLC(22.2%) and 3 of 26 SCLC(11.5%).Apoptosis index (AI) was negatively correlated with PCNA-labeling index (PCNA-LI)(r=-0.7358,P<0.01).There was a statistical difference of PCNA-LI between lung cancer with P-gp and without P-gp. P-gp was positively correlated with bcl-2 (r=0.5637,P<0.05),but not with P21 WAF1/CIPI(r=0.2465,P>0.05). Multivariate Cox regression analysis showed that P-gp and AI had prognostic significance.Conclusion P-gp correlates well with apoptosis?proliferation and apoptosis-related genes, and has prognostic significance.