| 急性髓系白血病细胞多药耐药相关基因的筛选 |
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| 引用本文： | 付劲蓉,刘文励,周剑锋,孙汉英,罗莉,冉丹,张恒. 急性髓系白血病细胞多药耐药相关基因的筛选[J]. 华中科技大学学报(医学版), 2005, 34(6): 659-662 |
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| 作者姓名： | 付劲蓉 刘文励 周剑锋 孙汉英 罗莉 冉丹 张恒 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院血液科,武汉,430030 |
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| 基金项目： | 中国博士后科学基金资助项目(No.2004036440) |
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| 摘 要： | 目的筛选急性髓系白血病细胞多药耐药相关基因。方法在建立HL-60／DNR多药耐药（MDR）细胞系的基础上，采用抑制消减杂交的方法结合基因芯片技术建立HL-60／DNR抑制消减杂交文库，并挑选其中部分克隆进行序列测定和同源性分析。结果HL-60／DNR对多种化疗药物均产生了不同程度的耐受。在所构建的HL-60／DNR抑制消减杂交文库中，经过基因芯片筛选出明显差异表达克隆共有14个；进一步分析发现这些基因在白血病细胞多药耐药中的作用大多未见报道。结论多种基因（已知的或未知的）均参与了急性髓系白血病细胞多药耐药的调节,大规模筛选与克隆这些基因为进一步研究急性髓系白血病细胞多药耐药产生的机制奠定了必要的理论基础.
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| 关 键 词： | 白血病 急性髓性 多药耐药 基因克隆 抑制消减杂交 基因芯片 |
| 修稿时间： | 2005-07-15 |
Screening and Cloning Multi-Drug Resistance Related Gene of Acute Myeloid Leukemia Cells |
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| Fu Jinrong,Liu Wenli,Zhou Jianfeng et al. Screening and Cloning Multi-Drug Resistance Related Gene of Acute Myeloid Leukemia Cells[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2005, 34(6): 659-662 |
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| Authors: | Fu Jinrong Liu Wenli Zhou Jianfeng et al |
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| Affiliation: | Fu Jinrong,Liu Wenli,Zhou Jianfeng et alDepartment of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective Try to screen and separate multidrug resistance(MDR) related genes in acute myeloid leukemia cell.Methods After establishing the MDR leukemia cell line (HL-60/DNR), a suppression subtractive hybridization library was established by suppression subtractive hybridization and gene chip.The resulting subtracted cDNA clones were partially sequenced and analyzed by blastn.Results The HL-60/DNR produced MDR to a variety of chemotherapeutic agents.14 genes were found to express 5-fold difference of expressioo levels between HL-60/DNR and untreated HL-60 cells and were selected to sequence. Most of the 14 sequenced genes have not been reported to be related to MDR in leukemia. Conclusion Many genes both known and unknown were found to relate to the MDR in AML. Discovery of these gene provided a solid foundation to elucidate the mechanism of MDR in acute myeloid leukemia cell. |
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| Keywords: | acute myeloid leukemia multi-drug resistance gene cloning suppression subtractive hybridization gene chip |
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