| 高效液相色谱法测定养胃宁胶囊中大黄酚含量 |
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| 引用本文： | 王英伟,于佳立,赵文倩,刘 莹,陈力研. 高效液相色谱法测定养胃宁胶囊中大黄酚含量[J]. 中国药业, 2012, 21(13): 29-30 |
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| 作者姓名： | 王英伟 于佳立 赵文倩 刘 莹 陈力研 |
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| 作者单位： | 长春卫尔赛生物药业有限公司,吉林长春,130616 |
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| 摘 要： | 目的 建立养胃宁胶囊中大黄酚的含量测定方法.方法 色谱柱为Phenomenex C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.1％磷酸(70∶30),流速为1.0mL/min,柱温为35℃,检测波长为254 nm.结果 大黄酚进样量在41.68～208.4 ng范围内与峰面积线性关系良好,回归方程为Y=5 484.98X-4 707.53(r=0.999 998),平均回收率为99.23％,RSD=1.37％(n=6).结论 所用方法简便、快捷、准确、专属性强,可用于养胃宁胶囊的质量控制.
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| 关 键 词： | 高效液相色谱法 大黄酚 含量测定 |
Content Determination of Chrysophanic Acid in Yangweining Jiaonang by HPLC |
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| Wang Yingwei , Yu Jiali , Zhao Wenqian , Liu Ying , Chen Liyan. Content Determination of Chrysophanic Acid in Yangweining Jiaonang by HPLC[J]. China Pharmaceuticals, 2012, 21(13): 29-30 |
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| Authors: | Wang Yingwei Yu Jiali Zhao Wenqian Liu Ying Chen Liyan |
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| Affiliation: | (Changchun Weiersai Biotech Pharmaceutical Co.Ltd.,Changchun,Jilin,China 130616) |
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| Abstract: | Objective To establish an HPLC method for the chrysophanic acid in Yangweining Jiaonang.Methods Chromatographic column is phenomenex C18（250 mm×4.6 mm,5 μm）,the mobile phase is methanol-0.1%phosphate（70 ∶30）;the flow rate is 1.0 mL/min;the column temperature is 35 ℃;the wavelength is 254 nm.Results The linear range of chrysophanic acid is 41.68-208.4 ng.Regression Equations is Y=-4 707.53＋5 484.98X,（r=0.999 998）.The average recovery is 99.23%,RSD=1.37%.Conclusion The method is simple,fast,accurate and specific,and it can be used for the quality control of Yangweining Jiaonang. |
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| Keywords: | HPLC chrysophanic acid content determination |
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