高效液相色谱法测定六味五灵片中齐墩果酸含量

目的 建立测定六味五灵片中齐墩果酸含量的高效液相色谱法.方法 色谱柱为Platisil ODS柱(250mm×4.6mm,5μm),流动相为甲醇-水-磷酸(80∶20∶0.4,V/V),流速为1.0mL/min,检测波长为220nm.结果 齐墩果酸质量浓度在0.325～2.600μg/mL范围内与峰面积线性关系良好,线性回归方程为A=2.2×105 C+3.90×1 03,r=0.999 9(n=7),平均回收率为99.71％,RSD=1.36％(n=9).结论 所用方法简便、重复性好,可用于六味五灵片中齐墩果酸的含量测定.

Determination of Oleanolic Acid in Liuweiwuling Tablet by HPLC

Objective To develop an HPLC method for the determination of oleanolic acid in Liuweiwuling Tablet.Methods The sample was separated on Platisil ODS column（250 mm×4.6 mm,5 μm） with methanol-water-phosphoric acid（80 ∶20 ∶0.4,V/V） as the mobile phase at a flow rate of 1.0 mL/min.The wavelength for detection was 220 nm and the column temperature was 50 ℃.Results The calibration curve was linear at a range of 0.325-2.600 μg/mL for the oleanolic acid.The regression equation was A=2.2×10^5C＋3.90×10^3（r=0.999 9）.The average recovery was 99.71%,and the related standard deviation（RSD） was 1.36%.Conclusion The method was proved to be simple reproducible and can be used for evaluation the quality of Liuweiwuling Tablet.