不同产地对九节菖蒲中总皂苷、齐墩果酸及腺苷含量的影响

目的测定不同产地九节菖蒲中总皂苷、齐墩果酸及腺苷的含量。方法以齐墩果酸为对照品,采用紫外分光光度法于208 nm波长处对九节菖蒲中的总皂苷含量进行测定;采用高效液相色谱法对九节菖蒲中齐墩果酸的含量进行测定,色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),柱温为室温(25℃),流动相为乙腈-水(92∶8),流速为1 mL/min,检测波长为208 nm;采用高效液相色谱法对九节菖蒲中腺苷的含量进行测定,色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm)柱,柱温为室温(25℃),流动相为乙腈-水(8∶92),流速为1 mL/min,检测波长为260 nm。结果不同产地九节菖蒲中总皂苷、齐墩果酸的含量差异较大,腺苷的含量差异较小。结论不同产地对九节菖蒲中总皂苷、齐墩果酸的含量有一定影响。

Influence of Producing Areas on Concentration of Total Saponins and Oleanolic Acid and Adenosine in the Roots of Anemzone Altaica

Objective To determine the level of total saponins and oleanolic acid and adenosine in the roots of Anemzone altaica from different habitats. Methods Using the content of total saponins as evaluation indexes by UV- 750. UV spectroscopy based on reference material of oleanolic acid,total saponins in the roots of Anemzone altaica were determined,detection wavelength was set at 208 nm. The concentration of oleanolic acid were determined by HPLC,Diamonsil C18 column （250 mm ×4.6 mm,5 um）,column temperature was at room temperature （25℃）,mobile phase of acetonitrile-water （92： 8）,flow rate of 1 mL/min, detection wavelength for 208 nm. The concentration of adenosine were determined by HPLC,Diamonsil C18 column （250 mm×4.6 mm,5 um）,column temperature was at room temperature （25℃）,mobile phase of acetonitrile-water-acetonitrile （8：92）,flow rate of 1 mL/min,detection wavelength of 260 nm. Results The contents of total saponins and oleanolic acid and adenosine in the roots of Anemzone altaica form different habitats were different from one another. Conclusion The contents of total saponins and oleanolic acid and adenosine in the roots of Anemzone altaica form different habitats were different.