三种不同方法检测结核分枝杆菌药物敏感性分析

目的探讨仪器液体比例法（BacT／ALERT 3D与MGIT960系统）与L—J比例法测试结核分枝杆菌药物敏感性结果的一致性。方法用BacT/ALERT3D快速药敏法、MGIT960快速药敏法、L—J比例法同时对107株结核分枝杆菌进行INH、SM、RFP及EMB四种抗结核药物的耐药性测定。结果（1）INH、SM、RFP及EMB用BacT／ALERT3D法、MGIT960法、L—J比例法药敏测试结果一致率分别为：93％、90％、95％、90％。（2）BacT／ALERT3D法、MGIT960法、L—J比例法对INH、SM、RFP、EMB的耐药率分别是：27％、28％、24％；12％、13％、19％；25％、25％、22％；7％、6％、9％。三种方法四种药物的耐药性均无显著性差异（P〉0．05）.（3）BacT／ALERT3D法、MGIT960法、L—J比例法完成药敏测试平均时间为10．5天、7．7天、28天，经统计存在差异显著性（P〈0．00）.结论BacT/ALERT3D法、MGIT960法、L-J比例三法对INH、SM、RFP、EMB药敏测试结果具有高的可比性。MGIT960法药敏检测时间最短。

Analyse of drug sensitivity of mycobacterium tuberculosis using three different methods

Objective To discuss the accordance of mycobacterium tuberculosis drug sensitivity test result with equipment liquid proportion method and L-J proportion method.Methods Detecting the four anti-mycobacterium drugs ( INH、 SM、 RFP, EMB ) susceptibility of 107 strains mycobacterium with BacT/ALERT 3D rapid drug sensitivity test 、MGIT 960 rapid drug sensitivity test and L-J proportion method. Results ( 1 ) The four drug ( INH、 SM、 RFP, EMB ) sensitivity ratio was 93%、 90%、 95% 、 90% respectively with BacT/ALERT 3D rapid drug sensitivity test、 MGIT 960 rapid drug sensitivity test and L-J proportion method.( 2 ) The resistance rate of INH、 SM、 RFP, EMB using BacT/ALERT 3D、 MGIT 960 and L-J proportion method was 27%、 28%、 24%; 12%、 13%、19%; 25%、 25%、 22%; 7%、 6%、 9% respectively.There was no significant difference among the drug resistance rate detected by the three methods.( 3 )The average drug sensitivity test time using BacT/ALERT 3D、 MGIT 960 and L-J proportion method was 10.5 days、 7.7 days、 28 days. There was significant difference through statistical analysis ( P ＜ 0.001 ) Conclusions The four drug ( INH、 SM、 RFP,EMB )sensitivity test using BacT/ALEBT 3D、MGIT 960 and L-J proportion method had high comparability.The drug sensitivity test time was shortest using MGIT 960 method.