桑皮苷A对照品的制备研究

目的 研究从桑白皮中制备桑皮苷A对照品的制备方法.方法 结合大孔树脂吸附、硅胶柱色谱和凝胶柱色谱对桑白皮提取物进行分离、纯化,利用TLC和HPLC-ELSD对分离产物进行质量分数检测,并通过各种波谱手段等对其进行结构确证.结果 从桑白皮中分离、纯化出桑皮苷A对照品,质量分数>98.5%.结论 该方法制备出的桑皮苷A对照品符合中药化学对照品的相关要求,可作为桑白皮药材和含桑白皮成药质量控制,以及中药药效物质基础研究用的化学对照品.

Preperation of reference substance of mulberroside A from root bark of Morus alba

Objective To establish a separation method for reference substance of mulberroside A from the root bark of Morus alba.Methods Ethanol-extract of the root bark of M.alba was isolated and purified by maeroporous resin,silica gel,and Sephdex LH-20 column chromatography.The purity of mul- berroside A was identified by TLC and ELSD-HPLC.Its structure was identified by spectral analysis.Re- sults The compound was completely separated from the root bark of M.alba.The purity of the reference substance was not less than 98.5%.Conclusion The compound prepared by the above-mentioned method is accorded with the relative demands of chemical reference substance in Chinese materia mediea.It can be used as a reference substance for the quality control and the research of herbal medicine.