| EMIT法与HPLC法测定人血浆中环孢素A浓度的比较研究 |
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| 引用本文： | 宋晓勇,张永州,吴先闯,王云香,吕维玲,杨磊.EMIT法与HPLC法测定人血浆中环孢素A浓度的比较研究[J].中国药房,2011(26):2443-2445. |
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| 作者姓名： | 宋晓勇 张永州 吴先闯 王云香 吕维玲 杨磊 |
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| 作者单位： | 河南大学淮河医院;河南大学药学院; |
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| 摘 要： | 目的:比较酶放大免疫(EMIT)法和高效液相色谱(HPLC)法测定人血浆中环孢素A(CsA)浓度的区别与相关性。方法:收集肾移植患者服药后的稳态谷浓度血样20份,分别用EMIT法和HPLC法进行测定,考察2种测定方法的区别和相关程度。结果:HPLC法和EMIT法检测的CsA血药浓度差异有统计学意义(P<0.05),EMIT法测定血浆中CsA浓度较HPLC法高26.4ng·mL-1,通过Passing-Bablok回归分析2种方法具有良好的相关性(r=0.9940)。结论:EMIT法和HPLC法测定CsA血浆药物浓度结果差异具有统计学意义,在临床进行CsA药物浓度监测中应予以关注并作相应调整。
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| 关 键 词： | 酶放大免疫法 高效液相色谱法 环孢素A 血药浓度 |
Determination of Plasma Concentrations of CsA by EMIT vs. HPLC |
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| SONG Xiao-yong,ZHANG Yong-zhou,WU Xian-chuang,WANG Yun-xiang,LV Wei-lingYANG Lei.Determination of Plasma Concentrations of CsA by EMIT vs. HPLC[J].China Pharmacy,2011(26):2443-2445. |
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| Authors: | SONG Xiao-yong ZHANG Yong-zhou WU Xian-chuang WANG Yun-xiang LV Wei-lingYANG Lei |
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| Institution: | SONG Xiao-yong,ZHANG Yong-zhou,WU Xian-chuang,WANG Yun-xiang,LV Wei-ling(Huaihe Hospital of He’nan University, Kaifeng 475000, China)YANG Lei(College of Pharmacy, Henan University, Kaifeng 475000, China) |
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| Abstract: | OBJECTIVE:To compare the difference and correlation of HPLC and enzyme-multiplied immunoassay test (EMIT) for the determination of blood concentration of CsA in human plasma. METHODS: A total of 20 different concentrations of clinical samples were collected and determined by HPLC and EMIT respectively. The difference and correlation of 2 kinds of determination methods were investigated. RESULTS: There were significant differences in blood concentrations of CsA determined by HPLC and EMIT(P<0.05). CsA concen... |
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| Keywords: | Enzyme-multiplied immunoassay test HPLC CsA Blood concentration |
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