HPLC法快速测定肺癌患者血浆中依托泊苷的浓度及其治疗窗考察

目的:建立快速测定肺癌患者血浆中依托泊苷浓度的方法,考察其治疗窗。方法:采用高效液相色谱法,色谱柱为Waters Symmetry C18,流动相为甲醇-水(45∶55),流速为1 mL·min-1,检测波长为220 nm,进样量为20 μL,柱温为30 ℃。用世界卫生组织(WHO)评价标准判定疗效及观察不良反应以考察治疗窗。结果:依托泊苷血药浓度在2.59～51.8 μg·mL-1范围内线性关系良好(r=0.999 7);方法回收率为94.5%～96.4%;日内、日间RSD均<5.0%。肺癌患者依托泊苷血药浓度治疗窗应为6.525～9.115 μg·mL-1。结论:本方法可快速、准确测定肺癌患者依托泊苷血药浓度;该药在治疗窗内安全、有效。

Rapid Determination of Plasma Concentration of Etoposide in Lung Cancer Patients and Investigation of Its Therapeutic Window

OBJECTIVE：To establish rapid method for the concentration determination of etoposide in plasma of lung cancer patients, and to investigate its therapeutic window. METHODS：HPIC method was adopted. The determination was performed on Waters SymmetryC18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-water （45∶55） at flow rate of 1 mL·min-1. The detection wavelength was set at 220 nm and injection volume was 20 μL. The column temperature was 30 ℃. The curative effect and result of adverse drug reaction observe were evaluated by WHO criteria to investigate therapeutic window. RESULTS： The linear range of etoposide was 2.59～51.8 μg·mL-1（r=0.999 7） with recovery of 94.5%～96.4%. The RSDs of intra-day and inter-day were all lower than 5.0%. The therapeutic window of etoposide in lung cancer patients wes 6.525～9.115 μg·mL-1. CONCLUSION： It’s quick and accurate to determine plasma concentration of etoposide in lung cancer patients. Etoposide is safe and effective in the range of therapeutic window.