皮肤与内脏相关的神经基础研究

目的在皮内注药治疗遗尿症及内脏痛有效的基础上,研究皮肤与内脏相关的初级神经元和交感神经元分布规律。方法通过家兔静脉注射伊文氏蓝(EvansBlue),胃内注入甲醛致胃伤害性刺激,观察皮肤渗漏斑,找出皮肤牵涉区;在牵涉区皮肤和胃黏膜分别注射辣根过氧化物酶(HRP)和荧光素核黄(NY)用神经逆行追踪法分别观察初级神经元和交感神经元分布规律。结果家兔胃痛的皮肤牵涉区在肩及肩胛区,在牵涉区皮肤和胃黏膜分别注射HRP和NY在C8～T8脊神经节和交感神经节相互重叠。结论胃痛的皮肤牵涉区与胃黏膜初级神经元分布的特点是在脊神经节呈节段性分布且相互重叠,在交感神经节呈弥散性分布,无节段性分布,也相互重叠,这可能是皮内注药治疗内脏痛的神经基础。

The basic study of nerves of skin associated with viscera

Aim To study the distribution regulation of primary neurons and sympathetic neurons of skin associated with viscera on the theoretical basis of treating visceral pain by intracutaneous injection drug.Methods By intravenously injecting Evans Blue dye,then injecting the formaldehyde into the stomach of rabbits for making the models of gastric noxious stimulation, the skin extravasation plots were observed and the skin referral area was found out.Moreover,the distribution regulation of primary neurons and sympathetic neurons were observed respectively by injecting HRP into the referral area and NY into the gastric mucosa respectively using neural retrograde tracing method.Results The pain referral area of rabbit was in the shoulder and scapular region.After injecting HRP into the referral area and NY into the gastric mucosa respectively,the C8- T8 spinal ganglion and sympathetic ganglion were superposed with each other.Conclusion The distribution characteristics of primary neurons in skin referral area and gastric mucosa are that spinal ganglions present segmental distribution and superposing with each other,moreover,sympathetic ganglions present diffused distribution and have no segmental distribution,which may be the neural basis of treating visceral pain by intracutaneous injection drug.