| 以HBc颗粒为呈现载体的真核表达载体pcDNA3．1-HBc^Hhel的构建 |
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| 引用本文： | 陈佳玉 李景利 赵文静 王海河 李凡. 以HBc颗粒为呈现载体的真核表达载体pcDNA3．1-HBc^Hhel的构建[J]. 中国现代医学杂志, 2006, 16(1): 54-56 |
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| 作者姓名： | 陈佳玉 李景利 赵文静 王海河 李凡 |
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| 作者单位： | [1]吉林大学基础医学院病原生物实验室,吉林长春130021 [2]博泰生物技术有限公司,吉林长春130021 [3]北方肝胆病医院,吉林长春130062 |
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| 摘 要： | 目的构建含乙肝核心C基因的真核表达载体pcDNA3．1-HBc^Hhel探索其作为DNA疫苗载体的可能性。方法采用分子克隆技术在原核表达质粒pTrc—core的HBcAg el—loop处添加Nhel酶切位点，双酶切此重组载体pTrc-core^Nhel及真核表达载体pcDNA3．1，产物经纯化、T4DNA连接酶连接，连接产物转化大肠杆菌DH5α，筛选抗Amp^＋克隆并提取质粒进行酶切及PCR鉴定。结果成功构建了真核表达质粒pcDNA3．1-HBc^Hhel。结论该重组质粒可作为治疗性及预防性疫苗的候选载体，为深入研究预防性及治疗性疫苗奠定了基础。
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| 关 键 词： | DNA疫苗 免疫反应 HBc基因 |
| 文章编号： | 1005-8982（2006）01-0054-03 |
| 收稿时间： | 2005-03-11 |
| 修稿时间： | 2005-03-11 |
Use HBc as carrier to construct expression-vector PCDNA3.1-HBc^Hhel |
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| CHEN Jia-yu, LI Jing-li, ZHAO Wen-jing, WANG Hai-he, LI Fan. Use HBc as carrier to construct expression-vector PCDNA3.1-HBc^Hhel[J]. China Journal of Modern Medicine, 2006, 16(1): 54-56 |
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| Authors: | CHEN Jia-yu LI Jing-li ZHAO Wen-jing WANG Hai-he LI Fan |
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| Affiliation: | 1.Department of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R.China; 2. Changchun Botai Biotechnology Limited Company, Changchun, Jilin 130021, P.R.China; 3. North Hepatobiliary Hospital, Changchun, Jilin 130062, P.R.China |
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| Abstract: | [Objective] To construct DNA vaccine vector pcDNA3.1-HBc^Nhel. [Methods] We usedPCR to add the NheI site to plasmid pTrc-core, then HBc^Nhel gene was cloned into vector pcDNA3.1, the target genes were sequenced and the plasmids were extracted. [Results] We successfully constrct the DNA vaccine vector. [Conclusion] The plasmid pcDNA3.1-HBc^Nhel might be able to serve as curing or preventive DNA vaccines candidate. |
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| Keywords: | DNA vaccine immune response HBc gene |
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