人类乳头瘤病毒在食管-胃交界处腺癌致病中的作用

目的 了解人类乳头瘤病毒(HPV)感染与交界于食管与胃之间的食管-胃交界处腺癌(AEG)发生的相关性.方法 共有106例AEG和60例胃癌患者被纳入本研究,其中包括14例AEGⅡ型和86例AEGⅢ型腺癌.每一位患者均留有可供研究的癌灶和癌旁黏膜组织.应用多聚酶链反应(PCR)检测的引物为高度敏感且识别谱广的SPF1和GP6+.PCR扩增产物经测序确认HPV感染并分型.结果 106例AEG患者和59例胃癌患者的基因组DNA达到检测标准.11例AEG患者和6例胃腺癌患者为HPV阳性,阳性率分别为10.4%和10.2%(P=0.966).AEG和胃癌患者在HPV总感染率方面差异无统计学意义.结论 HPV在AEGⅡ型、Ⅲ型以及胃腺癌的形成中并不发挥致病性作用.

Role of papillomavirus in adenocarcinoma of esophagogastric junction

Objective To determine if human paillomavirus (HPV) infection could be associated with the development of adenocarcinoma of esophagogastric junction (AEG). Methods A total of 106 consecutive AEG patients and 59 consecutive gastric carcinoma patients who accepted surgery at our department between January 2004 and December 2006 were selected. Specimens from tumors and uninvolved mucosa were obtained intra-operatively. Genomic DNA was extracted by the phenol-chloroform-isoamyl alcohol extraction protocol. The HPV infection was determined by PCR using primer set SPF1 and GP6 +.Specificity of the amplified products was confirmed by sequencing. Results The HPV infection rates in AEG and gastric cancer were 10.4% (11/106) and 10.2% (6/59) separately (P=0.966). Conclusion HPV is not a major factor in the carcinogenesis of AEG type Ⅱ , AEG type Ⅲ or stomach.