Rho A/Rho激酶信号通路参与慢性应激大鼠抑郁模型结肠动力的改变

目的:检测Rho A、Rock1、Rock2在抑郁大鼠结肠组织中mRNA及蛋白的表达,探讨Rho A/Rho激酶信号通路在抑郁大鼠结肠动力障碍中的可能机制.方法:通过慢性应激建立大鼠抑郁模型,正常组作对照,应用Real Time Q-PCR和Western blot检测Rho A、Rock1、Rock2在结肠组织中mRNA及蛋白的表达.结果:抑郁组结肠组织中的Rho A、Rock1、Rock2的mRNA及蛋白表达明显降低,与正常组比较差异有统计学意义(P＜0.01).结论:RhoA/Rho激酶信号通路在抑郁大鼠结肠动力障碍起着重要作用,但其作用机制仍需进一步的实验研究.

Rho A/Rho Kinase Signaling Pathway is Involved in the Functional Alteration of Colonic Motility of Rat Depression Models Induced by Chronic Stress

Objective: To measure the expression of Rho A,Rock1,and Rock2 in colon of depressive rat,and to explore the role of Rho A/Rho kinase signaling pathway in the functional alteration of colonic motility.Methods: Twenty adult male SD rats were randomly divided into depression model group(n=10,established by unpredicted chronic stress) and normal control group(n=10).Sucrose consumption test was performed before and after stress.Protein and mRNA expression of Rho A,Rock1,and Rock2 in colon were measured by Real Time Q-PCR and Western blot.Results: Compared with that respectively in the normal control group,the mRNA and protein expression of Rho A,Rock1,and Rock2 were significantly lower in depression model group(P<0.01).Conclusion: The Rho A/Rho kinase signaling pathway play a role in the functional alteration of colonic motility of rat depression models induced by chronic stress.