桔梗中总黄酮的提取工艺优化

目的优选从桔梗根、茎、叶中提取总黄酮的最佳工艺,并在最佳提取条件下测定桔梗根、茎、叶中总黄酮的含量。方法以总黄酮含量为指标,采用正交实验,考察了乙醇体积分数、超声时间和超声次数对总黄酮得率的影响。结果提取总黄酮的最佳工艺为A3B3C3,即乙醇体积分数70%,超声时间50min,超声次数3次。在最佳实验条件下,分别测得桔梗根、茎及叶中总黄酮的含量分别为0.027%,0.039%和0.26%。结论该工艺简单可行,是提取桔梗中总黄酮的有效途径。

Optimization of the extraction procedure of total flavonoids from Platycodon grandi f lorum (Jacq .) A .DC

Objective The research aims to optimize the extraction technology of total flavonoids and to determine the contents of to-tal flavonoids under the optimal extraction conditions from roots ,stems and leaves of Platycodon grandi f lorum （Jacq .） A .DC . Methods In the experiment ,total flavonoids were used as an indicator by using the orthogonal test to explore the effects of ethanol concentration ,ultrasonic time and ultrasonic frequency on the yield of total flavonoids .Results The results showed that the opti-mal extraction conditions were as follows ：extraction solvent was ethanol of 70% ,ultrasonic time was 50 min and ultrasonic fre-quency was three times .Under the optimum extraction conditions ,the contents of total flavonoids from roots ,stems and leaves were determined and the content was 0 .027% in roots ,0 .039% in stems and 0 .26% in leaves .Conclusion The process which is simple and feasible ,is effective to extract the total flavonoids from Platycodon grandi f lorum .