紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量

目的用紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量。方法采用AB-8、D-101、D-4020、HPD-400和NKA-95种大孔树脂对苣荬菜根部总三萜皂苷进行纯化,以齐墩果酸为对照品,50g·L-1香草醛-冰醋酸、高氯酸为显色系统,在546nm波长处测定总三萜皂苷含量。结果在AB-8、D-101、D-4020、HPD-400和NKA-9 5种树脂中,D-101对苣荬菜根部总三萜皂苷纯化效果最好;齐墩果酸质量浓度在0.034 2⁰.106 5mg·mL-1范围内,吸光度与含量呈良好的线性关系,回归方程Y=8.298 2 X-0.084 9(r=0.999 6),平均加样回收率为95.3%,RSD=1.5%(n=5),苣荬菜根部总三萜皂苷含量为0.202%,RSD=2.6%。结论该方法操作简单,快速灵敏,准确可靠,对设备要求不高,适用于苣荬菜根部总三萜皂苷的含量测定。

Determination of total triterpenes from the roots of Sonchus arvensis L .by spectro-photometry

Objective To establish a new method for the determination of total triterpenes from the roots of Sonchus arvensis L .by spectrophotometry .Methods By using AB-8 ,D-101 ,D-4020 ,HPD-400 and NKA-9 five kinds of macroporous resins for the pur-ification of total triterpenes from the roots of S .arvensis L .,with oleanolic acid as standard ,50 g · L -1 vanillin-acetic acid ,per-chloric acid as coloring systems ,triterpenes was measured by spectrophotometry and detected at 546 nm .Results D-101 macro-porous resin showed the best performance for the purification of the total triterpenes from the roots of S .arvensis L ..Absorbance and content of oleanolic acid showed a good linear relationship in the range of 0 .034 2-0 .106 5 mg · mL-1 .The regression equation was Y=8 .298 2X-0 .084 9（r=0 .999 6） ,and the average recovery rate was 95 .3% ,RSD=1 .5% （n=5） .The content of total triter-penes in the roots of S .arvensis L .was 0 .202% ,RSD = 2 .6% .Conclusion This method is simple ,fast and sensitive ,accurate and re-liable ,less demanding on the device ,and can be used for the content determination of total triterpenes from the roots of S .arvensis L ..