某院1 715例妇科就诊者人乳头瘤病毒感染率及基因型分布

目的分析妇科就诊人群人乳头瘤病毒(HPV)感染率及基因型分布，为宫颈癌的诊治及HPV疫苗的研制提供参考。 方法采用凯普医用核酸分子快速导流杂交基因芯片技术(Hybrimax)检测2009年11月至2016年10月宝鸡市中医医院门诊及住院的1 715例自愿接受生殖道HPV感染筛查且有性生活史妇女的21种HPV基因型别。 结果就诊人群宫颈感染HPV高危型以HPV58、HPV16、HPV52和HPV33型最为常见，而HPV低危型则以HPV11及CP8304为主。1 715例就诊者中266例HPV阳性，阳性率为15.51%。其中单一感染者168例(9.80%)，双重及多重感染者98例(5.71%)；就诊者以31～40岁和41～50岁年龄段HPV感染率较高，分别为18.31%和18.60%；其次为≤30岁就诊者，感染率为13.92%；>50岁患者HPV感染率较低(8.24%)，与≤30岁患者相比差异具有统计学意义(P均<0.05)。168例单一高危型HPV感染者以HPV58、HPV16、HPV52、HPV33型感染为主，分别为68例(40.48%)、40例(23.81%)、22例(13.10%)、10例(5.95%)，与HPV58型阳性率比较，HPV16、HPV52、HPV33型的阳性率差异均有统计学意义(P均<0.05)；低危型HPV感染者以HPV11型和CP8304感染为主，分别为16例(9.52%)和12例(7.14%)，差异无统计学意义(χ²= 1.512、P= 0.186)。98例双重及多重HPV感染者中双重感染者62例(63.27%)；三重感染者28例(28.57%)；四重感染者8例(8.16%)。双重及多重HPV感染亚型主要以HPV58、HPV16、HPV52和HPV11型为主，分别为25例(25.51%)、20例(20.41%)、16例(16.33%)和11例(11.22%)。 结论本院妇科就诊人群高危型HPV感染率基本符合亚洲人群分布规律，但存在一定区域性。

Distribution of infection rate and genotype of human papilloma virus among 1 715 gynecological patients in a hospital

ObjectiveTo investigate human papilloma virus (HPV) infection rate and type distribution in gynecological patients, and to provide reference for the diagnosis and treatment of cervical cancer and the development of HPV vaccine. MethodsTotal of 21 HPV genotypes of 1 715 women with HPV infection screening of reproductive tract and sexual history in Baoji Traditional Chinese Medicine Hospital from November 2009 to October 2016 were detected by Rapid Biotransformation Hybridization Gene Chip Technology (Hybrimax). ResultsHPV58, HPV16, HPV52 and HPV33 were the most common high risk types of cervical infection, while HPV 11 and CP8304 were the main risk types of HPV. Among the 1 715 patients, 266 cases were HPV positive, the positive rate was 15.51%. There were 168 cases (9.80%) with single infection and 98 cases (5.71%) with double and multiple infection, the infection rates of HPV were higher in patients aged 31-40 years old (18.31%) and 41-50 years old (18.60%), followed by those≤30 years old, the infection rate was 13.92%. The infection rate of HPV in patients > 50 years (8.24%) was significantly lower than that in patients≤30 years old (allP< 0. 05). The high risk type of single infection were HPV58, HPV16, HPV52 and HPV33 infection in 68 cases (40.48%), 40 cases (23.81%), 22 cases (13.10%) and 10 cases (5.95%), respectively. Compared with the positive rates of HPV58, the positive rate of HPV16, HPV52 and HPV33 type were significantly different (allP< 0. 05); HPV11 and CP8304 were the main types of infection in low risk patients (16 cases, 9.52%vs. 12 cases, 7.14%), but there was no significant difference (χ²= 1.512,P= 0.186). Among the 98 cases with double or multiple infection, 62 cases (63.27%) were double infection, 28 cases (28.57%) were triple infection, 8 cases (8.16%) were quadruple infection. The main subtypes of double and multiple infection were HPV58, HPV16, HPV52 and HPV11 for 25 cases (25.51%), 20 cases (20.41%), 16 cases (16.33%) and 11 cases (11.22%), respectively. ConclusionsThe infection rate of high-risk HPV of gynecological patients in our hospital basically accords with the distribution of Asian population, but with some regional characteristics.