钙通道阻滞剂对重度冻僵大鼠血液有形成分的影响

目的观察维拉帕米（verapanil）对冻僵大鼠血液有形成分的影响。方法实验动物为健康雄性wistar大鼠，随机分为5组，为对照组、冻僵组、干预1组、干预2组和干预3组。对照组于室温20℃下饲养，干预1、2、3组冷暴露前30min分别腹腔注射维拉帕米5、10和20mg／kg，冻僵组和干预组大鼠-30℃环境进行冷暴露80min后复温4h。观察血液WBC、RBC、PLT数量和肛温的变化。结果冻僵组和干预1组WBC数量明显高于对照组（P〈0．01），干预2组WBC数量高于对照组（P〈0．05），干预3组WBC数量与对照组比较，差异无统计学意义（P〉0．05）；各组红细胞数量差异无统计学意义（P〉0．05）；冻僵组PLT数量较对照组显著增高（P〈0．01），而干预组PLT数量与对照组比较，差异无统计学意义（P〉0．05）；冻僵组和干预组肛温均低于31℃，与对照组比较，温差差异P〈0．05，干预3组温差明显低于冻僵组（P〉0．05）。结论10mg／kg维拉帕米能有效调节重度冻僵大鼠的血液有形成分数量，改善血液循环，防止体温过低的副效应。

Effect of Calcium Channel Blocker on the Blood Visible Component of the Serious Frozen Stiff Rats

Objective] To observe the effects of verapamil on the blood visible component of the frozen stiff rats. Methods] Healthy male Wistar rats were randomly divided into five groups： control group, frozen stiff group, interventional group 1, interventional group 2 and interventional group 3. Control group was bred at the room temperature of 20 ℃ . Verapamil of 5, 10, 20 mg/kg were injected into the abdominal cavity of the rats of the Interventional groups 1, 2, 3 respectively. The rats of the frozen stiff and interventional groups were exposed to cold condition at the temperature of -30℃ for 80min and then rewarmed for 4 hours. The changes of the counts of WBC,RBC, PLT and the level of anus temperature were observed. Results ] The counts of the WBC of frozen stiff group and interventional group 1 were significantly higher than that of control group （P 〈0. 01 ） ; that of interventional group 2 was also higher than the control group （P 〈0. 05）; the difference of WBC count between interventional group 3 and control group was not significant （ P 〉 0. 05 ） ; The counts of the RBC between each 2 groups were not significantly different （ P 〉 0. 05 ） ; The counts of the PLT of frozen stiff group was significantly higher than that of control group （ P 〈 0. 01 ）, while that between the interventional groups and control group were no significant （ P 〉 0. 05 ）. The anus temperature levels of the frozen stiff group and interventional groups were all lower than 31 ℃, compared with the control group temperature the difference was significant （ P 〈0. 05 ） ; the temperature difference of interventional groups 3 were significantly lower than that of frozen stiff group（P 〈0. 05）. Conclusion] 10 mg/kg verapamil can effectively regulate blood visible component, improve blood circulation, and prevent the side effects of hypothermia in frozen stiff rats.