| Lipopolysaccharide induced hyper—and hypo—responsiveness in macrophage cell lines |
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| 引用本文： | 刘辉,孙为民,徐仁宝.Lipopolysaccharide induced hyper—and hypo—responsiveness in macrophage cell lines[J].中国人民解放军军医大学学报,2003,18(1):11-14. |
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| 作者姓名： | 刘辉 孙为民 徐仁宝 |
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| 作者单位： | [1]DepartmentofPathophysiology,CollegeofBasicMedicalSciences,SecondMilitaryMedicalUniversity,Shanghai200433,China [2]DepartmentofImmunology,CollegeofBasicMedicalSciences,SecondMilitaryMed |
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| 基金项目： | Supported by National Natural Science Foundation of China (No.39730210) |
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| 摘 要： | Objective:To build a cell model of LPS-induced hyper-and hypo-responsiveness in macrophage cells.Methods:Macrophage cell line RAW264.7 was pre-cultured with or without 10ng/ml LPS for 18h,then challenged with lipopolysaccharide(LPS)or MDP,Zymosan,PAF,FMLP,PMA for 24h.The levels of TNF-α，IL－1，IL－6，IL－10，NO and O2^- were measured.Results:LPS pretreatment markedly inhibited TNF-α NO and IL-6 production,but increased IL-1,IL-10 and O2^- release to LPS challenge.LPS pretreatment also altered macrophage responsiveness to the other stimuli.Conclusion:LPS can induce hyper-and hypo-responsiveness simultaneously in the macrophage cell lines.Changes in macrophage responsiveness depend on stimuli and effectors whic are measured.
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| 关 键 词： | 脂多糖 诱导 巨噬细胞 高反应性 低反应性 LPS 炎症反应 |
Lipopolysaccharide induced hyper- and hypo-responsiveness in macrophage cell lines |
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| LIU Hui SUN Wei-min,XU Ren-bao.Lipopolysaccharide induced hyper- and hypo-responsiveness in macrophage cell lines[J].Journal of Medical Colleges of PLA(China),2003,18(1):11-14. |
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| Authors: | LIU Hui SUN Wei-min XU Ren-bao |
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| Institution: | LIU Hui SUN Wei-min,XU Ren-bao
Department of Pathophysiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China Department of Immunology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433 |
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| Abstract: | Objective: To build a cell model of LPS-induced hyper- and hypo-responsiveness in macrophage cells .Methods: Macrophage cell line RAW264.7 was pre-cultured with or without 10 ng/ml LPS for 18 h, then challenged with lipopolysaccharide(LPS), or MDP, Zymosan, PAF, FMLP, PMA for 24 h.The levels of TNF-α , IL-1, IL-6, IL-10 , NO and O-2 were measured.Results: LPS pretreatment markedly inhibited TNF-α NO and IL-6 production, but increased IL-1, IL-10 and O-2 release to LPS challenge.LPS pretreatment also altered macrophage responsiveness to the other stimuli.Conclusion: LPS can induce hyper- and hypo-responsiveness simultaneously in the macrophage cell lines.Changes in macrophage responsiveness depend on stimuli and effectors which are measured. |
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| Keywords: | lipopolysaccaride(LPS) macrophage hypo-responsiveness hype-responsiveness |
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