| 一种新的真核细胞表达载体pEF－BOS的应用 |
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| 引用本文： | 刘崟,金伯泉.一种新的真核细胞表达载体pEF－BOS的应用[J].细胞与分子免疫学杂志,1996(2). |
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| 作者姓名： | 刘崟 金伯泉 |
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| 作者单位： | 第四军医大学免疫学教研室 |
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| 摘 要： | 采用一种新的真核细胞表达载体ｐＥＦ－ＢＯＳ在ＣＯＳ细胞内表达了ｇｐ１３０和ＧＡＭ两种分子，并利用免疫沉淀和Ｗｅｓｔｅｒｎ－ｂｌｏｔ观察了表达结果。发现应用ｐＥＦ－ＢＯＳ表达载体可在ＣＯＳ细胞内获得上述两种分子的较高表达，优于一般常用的ｐＳＶ表达载体。
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| 关 键 词： | 真核细胞 载体 表达 |
Transfection of COS cell using a new eukaryotic expression vector |
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| Liu Yin, Jin Boquan.Transfection of COS cell using a new eukaryotic expression vector[J].Journal of Cellular and Molecular Immunology,1996(2). |
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| Authors: | Liu Yin Jin Boquan |
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| Abstract: | A new eukaryotic expression vector-pEF-BOS was used in the transfection of COScell, and the expression was detected by Western-blot and immunoprecipitation. It was foundthat PEF-BOS could give rise to a rather high expression, much better than the commonly usedpSV vector. |
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| Keywords: | eukaryotic cell vector expression |
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