| 广东汉族人群HLA-Cw多态性及单体型分析 |
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| 引用本文： | 马红京 肖露露 尹晓林 郭坤元 叶欣. 广东汉族人群HLA-Cw多态性及单体型分析[J]. 第一军医大学学报, 2004, 24(8): 900-903 |
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| 作者姓名： | 马红京 肖露露 尹晓林 郭坤元 叶欣 |
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| 作者单位： | [1]第一军医大学珠江医院血液科，广东广州510282 [2]广州器官移植配型中心，广东广州510095 |
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| 摘 要： | 目的检测广东汉族人群HLA．Cw及HLA-A、B、DRB1基因频率，分析该人群HLA-Cw等位基因多态性及其单体型特点。方法骨髓移植供者185例，抗凝血提取DNA，半量全自动PCR-RSSO分型检测HLA-A、B、Cw、DRB1基因型。结果在分辨水平检出Cw等位基因11个，其中分布频率较高的依次为：Cw*03(0．2580)、Cw*07(0．1887)、Cw*01(0．1732)、Cw*08(0．1070)。统计分析呈现显著连锁不平衡的HLA-Cw．A单体型7个，HLA-Cw-B单体型20个，HLA—Cw．DRB1单体型10个。结论广东汉族群体HLA-Cw基因具有较为丰富的多态性，其与HLA-A、B、DRB1间连锁不平衡单体型具有地区性遗传特征。
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| 关 键 词： | 人类白细胞抗原-Cw 单体型 基因频率 多态性 |
HLA-Cw alleles polymorphism and haplotypes in Guangdong Han population] |
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| Hong-jing Ma,Lu-lu Xiao,Xiao-lin Yin,Kun-yuan Guo,Xin Ye. HLA-Cw alleles polymorphism and haplotypes in Guangdong Han population][J]. Journal of First Military Medical University, 2004, 24(8): 900-903 |
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| Authors: | Hong-jing Ma Lu-lu Xiao Xiao-lin Yin Kun-yuan Guo Xin Ye |
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| Affiliation: | Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. |
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| Abstract: | OBJECTIVE: To analyze the polymorphism and haplotypes of HLA-Cw and detect HLA-A, B, Cw and DRB1 allele frequencies in Guangdong Han population. METHOD: An auto semi-quantitative PCR with reverse sequence-specific oligonucleo- tide was adopted to explore the HLA-A, B, Cw and DRB1 genotypes of 185 bone marrow donors. RESULT: Eleven HLA-Cw alleles were obtained in which Cw*03 (0.2580), 07 (0.1887), 01 (0.1732), and 08 (0.1071) had much higher allele frequencies. A total of 7 HLA-Cw-A, 20 HLA-Cw-B and 10 HLA-Cw-DRB1 haplotypes were found. CONCLUSION: HLA-Cw alleles have richer polymorphisms and their linkage disequilibrium with HLA-A, B, DRB1 exhibits geographic genetic characteristics. |
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