酶联免疫吸附试验在流行性乙型脑炎血清学诊断上的应用

用ELISA法检测225份临床疑似乙脑患者血清抗体并将该法与CF进行了比较。ELISA法阳性155份,阳性率为63.9%; CF阳性75份,阳性率为33.3%。ELISA法几何平均滴度为1:20.2; CF为1:1.2。两种检测方法的结果呈正相关(r=0.56, P<0.001)。此外还检测了乙型肝炎、脊髄灰质炎患者的阳性血清和乙脑非疫区的其他患者血清,均未发现阳性反应。结果表明ELISA法敏感、特异、简便,只需少量试剂,结果读取客观,且所用试剂、器材都容易获得。因此这一技术可用于实验室诊断,且适于乙脑的流行病学调查研究。

Application of ELISA to Serologicai Diagnosis of Japanese B Encephalitis

An enzyme-linked immunosorbent assay(ELISA) was developed for detecting antibody to Japanese B encephalitis virus, and the results were compared with complement fixation (CF). A total of 225 sera were tested. 75 out of these were positive (33.3%) by CF, whereas 155 sera were positive (68.9%) by ELISA. Geometric mean titer was 1:20.2 by ELISA but it was 1:1.2 by CF. There was a positive correlation between ELISA and CF (r=0.56 p<0.001).We have examined sera of patients of other diseases including hepatitis B, poliomyelitis and in area without Japanese B encephalitis. All of these sera showed negative result. The above results suggest that the ELISA have been proven to be sensitive, specific and simple to perform. Therefore, this technique is suitable for laboratory diagnosis and serum-epidemiological study of Japanese B encephalitis.