复方双参合剂对小鼠耐缺氧作用的影响

目的观察复方双参合剂对常压及减压状态小鼠抗缺氧能力的影响。方法将50只小鼠随机分成5组,分别为空白组、丹参组、人参组、复方双参组和红景天组,每组10只。复制常压密闭缺氧模型,测定小鼠存活时间;复制减压缺氧模型,测定此环境下小鼠脑含水量,脑、心脏及血清中超氧化物歧化酶（SOD）、丙二醛（MDA）和乳酸脱氢酶（LDH）的含量。结果与空白组比较,常压密闭缺氧条件下,复方双参组可以显著延长小鼠的存活时间,比空白组延长了45.95%（P〈0.05）。减压缺氧条件下,除丹参组（76.07%）,其余给药组（人参组：74.03%,复方双参组：73.33%,红景天组：73.68%）与空白组（75.63%）相比脑含水量显著降低（P〈0.01）;给药组对小鼠脑部的SOD含量无明显作用,复方双参组（MDA：3.76 nmol/mL,LDH：3.44 IU/L）与空白组（MDA：6.13 nmol/mL,LDH：9.00 IU/L）和红景天组（MDA：4.87 nmol/mL,LDH：4.78 IU/L）相比可以显著降低小鼠脑部MDA和LDH含量（P〈0.01）;复方双参组（SOD：205.46 U/mL,MDA：1.31 nmol/mL）与空白组（SOD：138.55 U/mL,MDA：4.18 nmol/mL）相比,可以显著提高小鼠心脏SOD的含量（P〈0.01）并显著降低MDA含量（P〈0.01）;复方双参组（SOD：98.33 U/mL,LDH：5.27 IU/L,MDA：0.23 nmol/mL）与空白组（SOD：18.68 U/mL,LDH：1.98 IU/L,MDA：1.00 nmol/mL）相比,可以显著提高小鼠血清SOD和LDH的含量（P〈0.01）,并显著降低MDA含量（P〈0.01）,其效果优于阳性对照红景天组（SOD：54.13 U/mL,LDH：4.80 IU/L,MDA：0.15 nmol/mL）。结论复方双参合剂可以有效提高小鼠耐缺氧的能力。

Effects of the compound shuangshen on hypoxia tolerance of mice

Objective To study the effects of the compound shuangshen on hypoxia tolerance of mice on the normal pressure or lower pressure.Methods The 50 mice were randomly divided into 5 groups： blank control group,Danshen group,Renshen group,the compound shuangshen group and Rhodiola group.Each group had 10 mice.To establish the hypoxia model on the normal pressure,the survival time of mice were determined.To establish the hypoxia model on the lower pressure,the moisture capacity of mice brain were determined,and the superoxide dismutase（SOD）,malon dialdehyde（MDA） and lactate dehydrogenase（LDH） of mice brain,heart or blood serum were determined.Results Compared to blank control group,the compound shuangshen group could obviously increase the survival time of mice with the closed normobaric hypoxia,and the prolongation rate was 45.95%（P 0.05）.The moisture capacity of mice brain of administration groups（Renshen group： 74.03%,the compound shuangshen group： 73.33%,Rhodiola group： 73.68%） had significant decreased on the hypobaric hypoxia except Danshen group（76.07%）（P 0.01）,compared with blank control group（75.63%）.The brain SOD of administration groups had no change to blank control group.The brain MDA and LDH of the compound shuangshen group（MDA： 3.76 nmol/mL,LDH： 3.44 IU/L） obviously decreased compared with blank control group（MDA： 6.13 nmol/mL,LDH： 9.00 IU/L） and Rhodiola group（MDA： 4.87 nmol/mL,LDH： 4.78 IU/L）（P 0.01）.The compound shuangshen group（SOD： 205.46 U/mL,MDA： 1.31 nmol/mL） could signif icantly increase the heart SOD content（P 0.01） and significantly decrease the heart MDA content（P 0.01） than blank control group（SOD： 138.55 U/mL,MDA： 4.18 nmol/mL）.The compound shuangshen group（SOD： 98.33 U/mL,LDH： 5.27 IU/L,MDA： 0.23 nmol/mL） could significantly increase the blood serum SOD content or LDH content（P 0.01） and significantly decrease the blood serum MDA content（P 0.01） than blank control group（SOD： 18.68 U/mL,LDH： 1.98 IU/L,MDA： 1.00 nmol/mL）.The compound shuangshen on hypoxia tolerance of mice had better effective ness compared with Rhodiola group（SOD： 54.13 U/mL,LDH： 4.80 IU/L,MDA： 0.15 nmol/mL）.Conclusion The compound shuangshen can efficaciously improve the ability of hypoxia tolerance of mice.