聚乳酸-羟基乙酸胶原膜生物相容性的实验研究

目的　评价海绵状聚乳酸 羟基乙酸 (PLGA)胶原膜作为真皮支架材料的生物相容性。　方法　利用SD大鼠和培养的人表皮细胞、成纤维细胞 ,观察PLGA胶原膜在体内的降解过程和在体外的细胞毒性、溶血性以及与人体细胞的亲和性。　结果　PLGA胶原膜在体内具有适当的降解速率 ,9周可完全降解 ;置入皮下 3周后可见血管化 ,新生组织不断长入 ,未见明显的炎症反应。浸泡液对培养细胞没有明显的毒性作用 ,不发生溶血反应。成纤维细胞和表皮细胞在PLGA胶原膜上可快速黏附、增殖。　结论　PLGA胶原膜具有良好的生物相容性和细胞亲和性 ,具有适当的降解速率 ,符合作为组织工程支架材料的要求

Experimental studies on the biocompatibility of spongy PLGA-collagen membrane as a tissue-engineering dermal scaffold

Objective To evaluate the biocompatibility of spongy PLGA-collagen membrane as a dermal scaffold. Methods Adult SD rats,cultured human keratinocytes and fibroblasts were employed in the study. The in vivo degradation process and the in vitro cytotoxicity and hemolytic response of the PLGA-collagen membrane, and the cell affinity of the PLGA membrane with the human cells were investigatd. Results The PLGA-collagen membrane degraded completely in vivo at the 9th week with an acceptable speed, It was found that new tissues gradually grew into the pores of the matrix, and blood vessels could be the matrix at the 3rd week of implantation subcutaneously without obvious inflammatory reaction. The lixivium of the PLGA-collagen membrane did not induce apparent cytotoxicity and hemolytic response,and it could induce rapid adherence and proliferation of fibroblasts and keratinocytes on it. Conclusion The PLGA-collagen membrane posessed good biocompatibility cell affinity,with appropriate degradation speed, These properties seemed to meet the regairemeat of a tissue-engineering dermal scaffold.