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Diode array detection of low level co-eluting species in high-performance liquid chromatography |
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| Authors: | N H Anderson M R Gray and C J Hinds |
| Institution: | Sterling Research Group, Willowburn Avenue, Alnwick, Northumberland NE66 2JH, UK |
| Abstract: | A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant “difference spectra” allows for a qualitative judgement regarding the integrity of the peak under consideration. |
| Keywords: | Chromatographic peak integrity assessment diode array spectroscopy HPLC antazoline danazol co-eluting species |
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