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| The Effects of PDTC on Interleukin-1β-induced Nitric Oxide Production in Chondrocytes | |
| 引用本文： | 刘建湘,杜靖远,杨述华,邱晓华,刘日光,易诚青,李新春. The Effects of PDTC on Interleukin-1β-induced Nitric Oxide Production in Chondrocytes[J]. 华中科技大学学报(医学英德文版), 2009, 29(3): 300-303. DOI: 10.1007/s11596-009-0307-x |
| 作者姓名： | 刘建湘 杜靖远 杨述华 邱晓华 刘日光 易诚青 李新春 |
| 作者单位： | Jianxiang LIU,Jingyuan DU,Shuhua YANG,Xiaohua QIU(Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China);Riguang LIU(Department of Orthopedics, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China);Chengqing YI(Department of Orthopedics, Shanghai First Hospital, Shanghai 201620, China);Xinchun LI(Department of Orthopedics, Ningbo First Hospital, Ningbo 315010, China) |
| 摘 要： | In order to find new drugs to inhibit nitric oxide （NO） production, the effects of pyrrolidine dithiocarbamate （PDTC）, a nuclear factor-kappa B （NF-κB） inhibitor, on recombinant human interleukin-1β （rhIL-1β）-induced NO production in chondrocytes were investigated. Rat chondrocytes were isolated and cultured, divided into control, P0, P1, P2, P3 and P4 groups. The chondrocytes in the P0, P1, P2, P3 and P4 groups were treated with different concentrations of PDTC （0, 3, 10, 30, and 50 p.mol/L respectively） for 1 h and then incubated with 5 U/mL rhIL-1β for 24 h. NO assay kit and RT-PCR were used to detect the NO content and the iNOS mRNA expression in the chondrocytes The expression level of iNOS mRNA in control, P0, P1, P2, P3 and P4 groups was 0.02±0.01, 1.24±0.13, 1.21±0.14, 0.61±0.11, 0.40±0.09, 0.21±0.06, and the relative content of NO was 15.8±2.7, 100±14.8, 92.6±9.3, 68.3±14.2, 27.5±9.8, 19.8±3.6, respectively. In the P0, P1, P2, P3 and P4 groups, the expression of iNOS mRNA and NO production were significantly increased as compared with those in the control group. As compared with the P0 group, the expression of iNOS mRNA and NO content in control group were lower. In the P2, P3 and P4 groups, PDTC could significantly inhibit the expression of iNOS and NO production induced by rhIL-1β in a concentration-dependent manner. It is suggested that PDTC can inhibit NO production and iNOS mRNA expression induced by IL-1β, which may provide an alternative method for the treatment of osteoarthritis. |
| 关 键 词： | PDTC 骨科学 关节炎 治疗 |
The effects of PDTC on interleukin-1β-induced nitric oxide production in chondrocytes |
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| Jianxiang Liu,Jingyuan Du,Shuhua Yang,Xiaohua Qiu,Riguang Liu,Chengqing Yi,Xinchun Li. The effects of PDTC on interleukin-1β-induced nitric oxide production in chondrocytes[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2009, 29(3): 300-303. DOI: 10.1007/s11596-009-0307-x | |
| Authors: | Jianxiang Liu Jingyuan Du Shuhua Yang Xiaohua Qiu Riguang Liu Chengqing Yi Xinchun Li |
| Affiliation: | 1. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China 2. Department of Orthopedics, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China 3. Department of Orthopedics, Shanghai First Hospital, Shanghai 201620, China 4. Department of Orthopedics, Ningbo First Hospital, Ningbo 315010, China |
| Abstract: | Summary In order to find new drugs to inhibit nitric oxide (NO) production, the effects of pyrrolidine dithiocarbamate (PDTC), a nuclear factor-kappa B (NF-κB) inhibitor, on recombinant human interleukin-1β (rhIL-1β)-induced NO production in chondrocytes were investigated. Rat chondrocytes were isolated and cultured, divided into control, P0, P1, P2, P3 and P4 groups. The chondrocytes in the P0, P1, P2, P3 and P4 groups were treated with different concentrations of PDTC (0, 3, 10, 30, and 50 μmol/L respectively) for 1 h and then incubated with 5 U/mL rhIL-1β for 24 h. NO assay kit and RT-PCR were used to detect the NO content and the iNOS mRNA expression in the chondrocytes. The expression level of iNOS mRNA in control, P0, P1, P2, P3 and P4 groups was 0.02±0.01, 1.24±0.13, 1.21±0.14, 0.61±0.11, 0.40±0.09, 0.21±0.06, and the relative content of NO was 15.8±2.7, 100±14.8, 92.6±9.3, 68.3±14.2, 27.5±9.8, 19.8±3.6, respectively. In the P0, P1, P2, P3 and P4 groups, the expression of iNOS mRNA and NO production were significantly increased as compared with those in the control group. As compared with the P0 group, the expression of iNOS mRNA and NO content in control group were lower. In the P2, P3 and P4 groups, PDTC could significantly inhibit the expression of iNOS and NO production induced by rhIL-1β in a concentration-dependent manner. It is suggested that PDTC can inhibit NO production and iNOS mRNA expression induced by IL-1β, which may provide an alternative method for the treatment of osteoarthritis. |
| Keywords: | pyrrolidine dithiocarbamate chondrocyte nitric oxide nuclear factor-kappa B |
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