胃癌淋巴结转移与VEGF—D、VEGFR-3、LVD的关系

目的探讨胃癌淋巴结转移与血管内皮生长因子D（VEGF—D）、血管内皮生长因子受体3（VEGFR-3）、淋巴管密度（LVD）表达间的关系。方法对59例胃癌术后标本采用免疫组织化学sP法检测VEGF—D表达，用VEGFR-3抗体免疫组织化学sP法标记淋巴管，检测肿瘤组织及正常组织LVD。结果VEGF—D、VEGFR-3在癌组织中表达率分别为59．32％、67．80％，明显高于癌旁不典型增生组织及正常胃黏膜组织（P〈0．05）。癌周组织的LVD表达为（21．29±8．21），明显高于周边正常组织（P〈0．05）。VEGF—D表达阳性组35例与阴性组24例的LVD分别为（23．15±7．58）和（11．93±5．31），其差异具有统计学意义（P〈0．05）。VEGF—D、LVD在癌组织中的表达与淋巴结直径、淋巴结分期、浸润深度、TNM分期及分化程度有关（P〈0．05）。结论VEGF．D在胃癌组织中高表达，并与肿瘤的淋巴管形成及淋巴结转移密切相关。

Correlation of gastric cancer in lymph node metastasis among the pathology features and the expression of VEGF-D, VEGFR-3, LVD

Objective To investigate the relationship of gastric cancer in lymph node metastasis among the pathology features and the expression of vascular endothelial growth factor-D （VEGF-D） , vascular endothelial growth factor receptor- 3 （ VEGFR-3 ） , lymphatic vessel density （LVD）. Methods The protein expression of VEGF-D, VEGFR-3, LVD was detected by immunohistochemical SP technique in 59 gastric cancer specimens. Results The positive rates of VEGF-D, VEGFR-3 in gastric carcinoma tissues were 59.32% ,67.80% respectively, which were significantly higher than those in a- typical hyperplasia tissues of adjacent carcinoma and normal gastric mucous membrane tissues （P 〈 0. 05 ）. The expression of LVD in adjacent carcinoma tissues was （21.29 ± 8.21 ）, which was significantly higher than that in adjacent normal tissues. LVD was （ 23.15 ± 7.58 ） , （ 11.93 ± 5.31 ） respectively in the 35 cases with positive expression and 24 cases with negative expression of VEGF-D. The differences were statistically significant in each of the groups （ P 〈 0.05 ）. The expressions of VEGF-D and LVD were correlated with pathologic features including lymph node size, lymph node metastasis staging, serosal invasion staging （ P 〈 0.05 ）, TNM staging and the degree of differentiation. Conclusion The expression of VEGF-D is higher in the gastric carcinoma tissues, and it is closely correlated with the lymphatic vessel development and lymph node metastasis of gastric carcinoma.