疏肝健脾法调控肠易激综合征动物模型胃肠激素水平的研究

目的:观察疏肝健脾法对肠易激综合征动物模型胃肠激素水平的影响。方法:将32只Wistar大鼠随机分为正常对照组、模型组、痛泻要方组、得舒特组。采用束缚应激刺激加灌服番泻叶煎剂的方法造成肠易激综合征(IBS)大鼠模型,观察各组大鼠稀便率,以放射免疫法检测各组大鼠血浆及局部肠组织中生长抑素(SS)、P物质(SP)水平的变化。结果:痛泻要方组经治疗后,稀便率下降,与模型组比较有统计学意义(P<0.05);模型组大鼠血浆和局部肠组织中SS、SP水平较正常对照组明显升高(P<0.05,P<0.01);痛泻要方组血浆及局部肠组织中SS、SP水平较模型对照组明显降低(P<0.05)。结论:疏肝健脾法对IBS动物模型胃肠激素有调控作用,能降低IBS动物模型体内SS、SP的分泌释放水平。

Study on the Method of Soothing the Liver and Strengthening the Spleen on Regulating Gastrointestinal Hormones in Animals Model of Irritable Bowel Syndrome

Objective:To observe the effects of the method of soothing the liver and strengthening the spleen on gastrointestinal hormone somatostain(SS) and substance P(SP) in animals model of irritable bowel syndrome(IBS).Methods:32 Wistar rats were divided randomly into normal control group,model group,Tongxieyaofang group and dicetel group.The rat model of irritable bowel syndrome(IBS) was made by binding stress with taking senna decoction.Changes of diarrhea rate were observed in these groups.Radioimmunoassay was used to detect the level of SS and SP in plasma and intestinal tissue in rats of each group.Results:The diarrhea rates in Tongxieyaofang group were decreased after treatment,and had the statistics significance in comparison with model group.The contents of SS and SP in plasma and intestinal tissue in model group were higher than those in normal group(P<0.05,P<0.01).The levels of plasma and intestinal tissue SS and SP in Tongxieyaofang group were markedly decreased than those in model group(P<0.05).Conclusions:The method of soothing the liver and strengthening the spleen had the effect of regulating gastrointestinal hormones and reducing the levels of SS and SP in animal model of IBS.