中医不同治法对实验性高脂血症兔主动脉内膜脂斑形成的影响

本文利用动物模型,以形态学为主,比较研究了活血化瘀(水蛭、川芎)、健脾化痰(半夏、陈皮、茯苓、竹沥水、甘草)和痰瘀同治(将上述两方合并而成)3种中医治法对实验性高脂血症家兔主动脉内膜脂斑形成及脂质水平的影响。发现3者均能显著地降低家兔血清TC、TG、LDL—C和LPO含量和抑制主动脉内膜脂斑的形成,其中活血化瘀组的作用强度与西药安妥明接近。然而化痰组在降低血清TC、LDL—C和抗脂斑形成方面均比活血化瘀组明显。3种治法中,以痰瘀同治组的作用最明显,与活血化瘀组及安妥明组相比其降低血清TC、LDL—C和抗脂斑形成的作用有非常显著的差异。其抗脂质过氧化作用也有明显差异,并稍优于化痰组。形态学观察与生化测定结果,与血清LDL-C及LPO含量变化相一致,即血中TC、LDL-C含量越高,LDO的浓度则越高,脂斑形成百分率就越高,对脂斑病变程度的镜下观察也显示了相同的对应关系。表明脂质代谢紊乱,血中TC、TG及LDL-C含量的升高,导致LPO的累积是血管内膜受损及脂斑形成的主要原因。

Influence of different TCM therapies on formation of fat plaque in aortic intima of experimental hyperlipi-demic rabbit

Three kinds of TCM therapy, i.e. blood- activating stasis femoving (leech. Rhizoma Ligustici chuanx-iong. 1st group), spleen- strenthening and phleg-mdissipating (Rhizoma pinellia. Poria tangerine peel. Radix Glycyrrhiza. and fluid of bamboo sap 2nd group) and simultaneous therapy on phlegm and stasis (3rd group), were applied morphologically in animal model to observe their influence on the lipid level and lipid plaque formation in the aortic intima of experimental hyper lipidemic rabbit. Results revealed that all 3 methods markedly lowered the serum TC, TG. LDL-C and LPO contents and inhibited the formation of aorticintimal lipid plaque. Its action in the 1st group and that in the atromid - s group were similar. However, the lowering of serum TC. LDL- C and ami-formation of lipid plaque in the phlegm dissipating group was better in the 2nd or blood-activating stasis removing group. In all the 3 groups, the 3rd group revealed the best results. When compared with the 1st group and atromid - S group, the difference was very significant in lowering of serum TC. LDL - C and anti -formation of lipid plaque, and was also a bit better than the 2nd group, morphological and biochemical studies revealed that it changed identically with the changes in serum LDL - C and LPO. or. in other words, the higher the contents of blood TC. LDL -C. the higher the concentration of LPO. and the higher the percentage of plaque formation. The degree of plaque formation under the microscope revealed the same correlative changes, suggesting that the accumulation of LPO as a result of lipid metabolic disturbance, increase of blood TC. TG. LDL - C contents were the major causes of injury of arterial intima and lipid plaque formation.