p21在电离辐射诱导EL-4细胞G_1期阻滞中的作用

目的　探讨 p2 1在电离辐射诱导EL 4细胞G1期阻滞中的作用。方法　采用North ernblot检测 p2 1WAF1mRNA水平的变化 ;采用流式细胞术检测 p2 1蛋白表达及细胞周期的变化。结果　 4 0GyX射线照射后 12h、2 4h、48hG1期EL 4细胞百分数明显高于假照射组 ;p2 1WAF1mRNA水平从照射后 1h开始升高 ,4h达峰值 ,持续至照后 12h ;p2 1蛋白表达在照射后 2～ 48h明显增高。结论　 4 0GyX射线照射可诱导EL 4细胞G1期阻滞 ,p2 1在电离辐射诱导EL 4细胞G1期阻滞中起重要作用。

The role of p21 in G_1 arrest of EL-4 cells induced by ionizing radiation

Objective To elucidate the role of p21 in G 1 arrest of EL-4 cells induced by ionizing radiation. Methods\ Northern blot was used to measure p21 WAF1 mRNA level.Flow cytometry was employed to analyze p21 protein expression and cell cycle of EL\|4 cells. Results\ Following 4\^0 Gy X\|irradiation,EL\|4 cells in G 1 phase began to significantly increase at 12 h,reached the peak at 24 h,and kept at high level till 48 h;the p21 WAF1 mRNA level began to increase at 1 h,reached the peak level at 4 h,kept the high levels till 12 h,then recovered to normal at 24 h;and the level of p21 protein expression in EI\|4 cells increased significantly at 2 h to 48 h. Conclusion\ The results suggest that p21 might play an important role in G 1 arrest of EL\|4 cells induced by ionizing radiation.