| ras-raf信号转导途径对受辐射细胞G_1期阻滞的调控作用 |
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| 引用本文： | 郭德煌,董波,刘农乐. ras-raf信号转导途径对受辐射细胞G_1期阻滞的调控作用[J]. 中华放射医学与防护杂志, 2000, 20(6) |
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| 作者姓名： | 郭德煌 董波 刘农乐 |
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| 作者单位： | 北京放射医学研究所(郭德煌!100850，董波!100850，文耕云!100850，罗庆良!100850，毛秉智!100850)，北京基础医学研究所(刘农乐) |
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| 基金项目： | 军事医学科学院博士后基金资助项目!( 2 0 10 1) |
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| 摘 要： | 目的 探讨ras raf信号转导途径在受辐射KG 1细胞中对周期的调控作用方式。方法 通过转染DN ras阻断GM CSF的信号传递后 ,瞬间转染cyclinD1,观察cyclinD1对受辐射细胞周期阻滞的影响作用。结果 转染DN ras的受辐射KG 1细胞即使用GM CSF刺激亦不能跳出G1期阻滞 ;瞬间转染cyclinD1能促进受辐射细胞跳出周期阻滞。 结论 ras raf信号转导途径通过促进周期蛋白cyclinD1的表达而对受辐射细胞周期进行调控。
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| 关 键 词： | 辐射 细胞周期 周期蛋白D1 |
The regulation of ras-raf signaling pathway on G_1 phase of the irradiated cells |
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| GUO Dehuang,DONG Bo,LIU Nongle ,et al.. The regulation of ras-raf signaling pathway on G_1 phase of the irradiated cells[J]. Chinese Journal of Radiological Medicine and Protection, 2000, 20(6) |
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| Authors: | GUO Dehuang DONG Bo LIU Nongle et al. |
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| Affiliation: | GUO Dehuang,DONG Bo,LIU Nongle *,et al. Institute of Radiation Medicine,Beijing 100850,China, *Institute of Basic Medicine,Beijing 100850,China, |
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| Abstract: | Objective To investigate the way of ras|raf signaling pathway which regulate the G 1 phase in irradiated KG|1 cells. Methods Blocked the GM|CSF signaling pathway by transfected DN|ras and then momentary transfected cyclin D1 into irradiated KG|1 cells,the effects of cyclin D1 on G 1 phase was examined. Results The irradiated KG|1 cells transfected DN|ras can't recover form G 1 phase arrest even though the GM|CSF was given,momentary transfected cyclin D1 promote the irradiated KG|1 cells from G 1 arrest. Conclusion Activation of ras|raf signaling pathway regulate the cell cycle of the irradiated KG|1 cells through promotion the expression of the cyclin D1. |
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| Keywords: | Irradiation Cell cycle Cyclin |
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