新型镇静催眠化合物B2对小鼠脑内单胺类神经递质水平的影响

目的研究新型镇静催眠化合物B2对小鼠脑内单胺类神经递质水平的影响。方法小鼠随机分为溶剂对照组和B2给药组（5mg／kg，ip），采用高效液相色谱-电化学法（HPLC-ECD）检测B2对小鼠不同脑区中去甲肾上腺素（NE）、多巴胺（DA）和5-羟色胺（5-HT）等单胺类神经递质水平的影响；并用比色法测定化合物B2对小鼠下丘脑、纹状体及大脑皮质中单胺氧化酶（MAO）活性的影响。结果与对照组相比，B2（5mg／kg，ip）可使小鼠下丘脑中NE含量由（1.08±0．09）μg／g显著降低至（0．88±0．07）μg／g（P〈0．01）；纹状体中DA含量由（12．85±2．21）μg/g显著降低至（8．44±2．25）μg／g（P〈0．01）；下丘脑中5-HT含量由（1．12±0．12）μg／g显著降低至（0．56±0．18）μg／g（P〈0．01）。B2对小鼠不同脑区MAO的活性无显著影响。结论B2引起小鼠不同脑区中单胺类递质NE、DA和5-HT水平的下降可能是其发挥镇静催眠作用的机制之一。

Effect of a novel sedative and hypnotic compound B2 on monoamine neurotransmitters levels in mouse brain

Objective To study the effect of sedative and hypnotic compound B2 on levels of monoamine neurotransmitters, including norepinephrine （NE） , dopamine （DA） , and 5-hydroxytryptamine （5-HT） in mouse brain. Methods Mice were randomly divided into vehicle group and B2 group （5 mg/kg, ip）, then the contents of NE, DA and 5-HT were detected by high performance liquid chromatography-electrochemical detection （HPLC-ECD）. The effects of compound B2 on monoamine oxidase （MAO） activities in mouse hypothalamus, striatum and cortex were detected by colorimetrie method. Results B2 （5 mg/kg, ip） could significantly re- duce NE contoent in the hypothalamus from （ 1.08 ± 0. 09 ） μg/g to （0. 88 ± 0. 07 ）μg/g （ P 〈 0.01 ） and reduce DA content in the striatum from （ 12. 85 ± 2. 21 ） μg/g to （ 8.44 ± 2. 25 ） μg/g （ P 〈 0. 01 ）. 5-HT content in hypothalamus were significantly decreased from （ 1.12 ±0. 12）μg/g to （0. 56 ±0. 18） μg/g （P 〈0. 01 ）. There was no significant difference between two groups in MAO activi- ty. Conclusion The sedative and hypnotic effects of B2 might be mediated by the decrease of monoamine levels in different mouse brain regions.