急性白血病红细胞CD55+、CD59+分子的检测及意义

目的:探讨急性白血病(AL)红细胞CD55 、CD59 表达及其临床意义。方法:AL患者42例,正常对照组42例,采用免疫荧光流式细胞仪检测红细胞膜表面CD55 、CD59 分子数量,采用全自动蛋白分析仪检测体液免疫相关指标。对AL患者,按化疗前后骨髓中原始细胞的百分数,将其分为缓解组和未缓解组,并对2组患者的红细胞膜表面CD55 、CD59 分子检测结果进行比较。结果:AL患者红细胞膜表面CD55 、CD59 分子数量较正常对照组下降,差别有统计学意义(P均<0.05);CD55 分子与IgA呈正相关(P<0.05);CD59 分子与补体C3、C4呈负相关(P<0.05);CD55 分子数量在AL患者未缓解组较缓解组下降,差别有统计学意义(P<0.05)。结论:AL患者红细胞天然免疫功能下降;红细胞膜表面CD55、CD59分子参与体液免疫反应调控,但CD55 分子的数量能否作为判断AL患者免疫状态及预后的指标之一,有待进一步探索和研究。

Detection and significance of CD55+ and CD59+ in the erythrocyte and body fluid immunity sign with acute leukemia

Objective: To explore the expression and clinical significancy of erythrocyte CD55 and CD59 in patients with acute leukemia(AL).Methods: The amount of CD55 and CD59 on the blood cell in 42 patients with acute leukemia and 42 normal by immune fluorescent light flow cytometery were measured.By full automatic protein analyze appearance detect the relational norm on body fluid immune.The patients with AL were seperated into two groups of mitigator and not mitigator according to the situation of marrow mitigated,before and after chemotherapy CD55 ,CD59 molecule on red cytomembrane surface detecting result of 2 parts of patients with AL were analysed.Results: The amount of CD55 and CD59 in the patients with AL significantly decreased than of normal controls.There were significant differences between two groups(P<0.05).There was a positive correlation between the amount of CD55 and IgA(P<0.05).There was a negative correlation between the amount of CD59 and C3,C4(P<0.05).The amount of CD55 significantly decreased between the groups of mitigator and not mitigator in patients with AL.The difference had significant meaning(P<0.05).Conclusion: The immune function of erythrocytes in patients with AL is depressed.Red blood cell CD55 and CD59 member have participated body fluid immune response regulation.Whether did CD55 take judges one of the AL patient immunity condition and pre-after targets,waits for further explores and research.