复合膳食纤维对大鼠生长发育和红细胞膜流动性的影响

目的 :　制备复合膳食纤维 (AFC) ,并分别探讨其与三种单一的膳食纤维 (DF)对高脂血症大鼠生长发育和红细胞膜流动性的影响。方法 :　健康、雌性、断乳 Wistar大鼠 48只 ,按体重随机分为 6组 ,用高脂饲料诱发高脂血症的同时 ,分别添加 1 0 %的 DF:纤维素、果胶、海藻酸钠、(纤维素 -果胶复合膳食纤维 ) AFC1、(纤维素 -海藻酸钠复合膳食纤维 ) AFC2 ,高脂饲料组 (对照组 ) ,观察各种 DF对大鼠的生长发育、粪脂排出量及脂质过氧化、红细胞膜流动性的影响。结果 :1 .添加 1 0 %的各种 DF不影响大鼠的生长发育。 2 .各种 DF可不同程度地增加粪重和粪脂排出量(P<0 .0 5 ,除海藻酸钠组外 ) ,以 AFC为明显。 3 .各种 DF皆可显著升高血清超氧化物歧化酶(SOD) (P<0 .0 1 )、谷胱甘肽过氧化物酶 (GSH-Px)活性 (P<0 .0 1 ) ,降低丙二醛 (MDA)水平 (P<0 .0 5 ,除纤维素、海藻酸钠组外 ) ,提高红细胞膜的流动性 (P<0 .0 5 ) ,以 AFC为明显。结论 :　各种 DF皆可不同程度地增加粪脂排出量、提高红细胞膜的流动性 ,其中可溶性 DF效果优于不可溶性 DF,而 AFC的效果又优于单一的 DF。

EFFECT OF ATIFICIAL FIBER COMPLEXES ON GROWTH AND ERYTHROCYTE MEMBRANE FLUIDITY IN RATS

Objective: To examine the effect of artificial fiber complexes (AFC) on growth and RBC membrane fluidity in comparison with three single fibers. Methods: 48 healthy, female Wistar rats (50-80 g) were randomly divided into 6 groups based on body weight. All five kinds of DF (cellulose, pectin, sodium alginate, cellulose pectin complex, cellulose sodium alginate complex) were given at a level of 10% in a lipid rich diet. The control group was fed lipid rich diet alone without DF. After 8 weeks, the effect on growth and development, lipid peroxidation, RBC membrane fluidity were studied. Results: 1.All DF groups did not affect the growth and development of the rats. 2.The fecal weight and its fat content in all DF groups were significantly higher than that in control group, especially for AFC group.3. All kinds of DF could increase serum SOD and GSH Px activities and reduce MDA content (except cellulose and sodium alginate group). RBC membrane fluidity was significantly increased in DF groups, and AFC groups were the most effective. Conclusion: All these kinds of DF have the effect of increasing RBC membrane fluidity, and AFC is the best.