A new, simple and rapid method for enumerating human T lymphocytes in full blood: the E. coli (ATCC 11303) rosette test |
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Authors: | E. Orye Y. Benoit L. Roesbeke M. Van Yper M. De Wilde |
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Affiliation: | Laboratory of Cellular Immunology, Department of Pediatrics, Rijksuniversiteit Gent, De Pintelaan 185, B-9000, Gent, Belgium |
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Abstract: | A new bacterial rosette technique for enumerating T lymphocytes is described. E. coli (strain B; ATCC 11303), fixed in formaldehyde after overnight growth in thioglycolate medium, are mixed with washed whole blood cells (100 μl) and after incubation at 4°C, slides are made, stained and counted. The nature of the lymphocytes forming E. coli rosettes was demonstrated by comparing their cytochemical staining characteristics with those of E rosetted lymphocytes, and by mixed E. coli and E, mouse E rosette and Fc receptor tests, and by mixed E. coli rosette tests and anti-Ig staining. E. coli and E rosette tests in controls and pediatric patients were also compared. The results show that Tμ and Tγ cells rosette with E. coli. |
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Keywords: | E. coli rosette test human Tμ and Tγ cells T cell surface markers |
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