蚬壳花椒果皮多糖抗肿瘤活性研究

目的:研究蚬壳花椒果皮多糖的体内外抗肿瘤活性。方法:采用水提醇沉法制备蚬壳花椒果皮多糖,以MTT法和移植性H22实体荷瘤小鼠为模型,检测不同剂量蚬壳花椒果皮多糖的体内外抗肿瘤作用。结果:当质量浓度为62.5～1 000μg/mL时,其对体外培养的2株人肿瘤细胞Hela和SMMC-7721的增殖表现出显著的抑制作用,IC50依次为460.9μg/mL、425.6μg/mL。体内实验表明,蚬壳花椒果皮多糖高(1.5g/kg)、中(0.75g/kg)、低(0.375g/kg)剂量组均能抑制荷瘤小鼠瘤块的生长,抑瘤率依次为41.55%、34.96%、33.71%;通过肿瘤组织病理学切片观察,同样显示蚬壳花椒果皮多糖具有较好的抗肿瘤作用。比较给药后荷瘤小鼠的肝脏指数、脾脏指数和胸腺指数后发现,蚬壳花椒果皮多糖毒副作用小,增强免疫功能。结论:蚬壳花椒果皮多糖在体内外均有明显的抗肿瘤活性。

Anti-tumor Activities of Total Polysaccharides from Zanthoxylum Dissitum Pericarp

Objective ： To observe the anti - tumor activity of the total polysaccharides from Zanthoxylum dissitum pericarp in vitro and vivo. Methods ： Its anti - tumor activity in vitro and in vivo was evaluated by MTF colorimetric assay and H22 cells -bearing mice models. Results： The results showed that the total polysaccharides from Zanthoxylum dissitum peri- carp significantly inhibited proliferation of the human cervical carcinoma cell Hela and the human hepatoma cell lines SMMC -7721 at the concentration of 62.5 -1 0001μg/mL, and the ICso value on these two tumor cells were 460. 9μg/ mL and 425.61μg/mL, respectively. In vivo, high （ 1.5 g/kg）, middle （0.75 g/kg） and low （0. 375 g/kg） dose total poly- saccharides from Zanthoxylum dissitum pericarp also significantly suppressed the tumor growth with tumor inhibition rate of 41.55%, 34.96% and 33.71% respectively. The Slice through the pathological observation of tumor tissue also indi- cated that it had a significant anti - tumor activity. Meanwhile, the results indicated the total polysaccharides from Zan- thoxylum dissitum pericarp could display certain strengthened action to the immune organ of tumor - bearing and did not have other side effects on tissues of mice. Conclusion： The total polysaccharides from Zanthoxylum dissitum pericarp possesses antitumor effects both in vitro and vivo.