褪黑素对庆大霉素急性肾损伤保护作用的研究

目的观察褪黑素（MLT）对庆大霉素（GM）所致大鼠急性肾损伤的保护作用,并探讨其可能的机制。方法将24只Wistar大鼠随机分为GM组、GM＋MLT组、对照（Cont）组。应用药物前后,分别记录并观察各组大鼠体质量和24 h尿量的变化;检测并观察用药后第5天血尿素氮（BUN）、肌酐（Cr）、尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）以及血清、组织匀浆中丙二醛（MDA）、过氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSH-Px）各指标的变化;处死大鼠后经肉眼观察和光镜检查肾组织的形态学变化。结果3组大鼠的肾组织形态学评分比较,差异有显著性;GM＋MLT组血BUN、Cr,尿NAG以及血清、组织匀浆中MDA、SOD、GSH-Px各指标与GM组比较差异有显著性（t=-10.41-9.55,P〈0.01）。结论MLT可通过抗氧化作用减轻庆大霉素对肾脏的损伤,对急性肾损伤具有一定的保护作用。

THE PROTECTIVE EFFECT OF MELATONIN ON GENTAMICIN-INDUCED ACUTE RENAL INJURY

ObjectiveTo study whether melatonin possesses protective effects on gentamicin-induced acute renal injury and approach its possible mechanism.MethodsTwenty-four adult Wistar rats were randomly divided into three groups: GM group,GM+MLT group and Control group.The weight and urine volume were recorded before and after the experiment.Blood urea nitrogen(BUN),serum creatinine(Cr),the level of malondialdehyde(MDA) in serum and renal tissue,the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in serum and renal tissue were measured before and five days after gentamicin and melatonin were administered.Renal histomorphological changes were observed through naked eyes and microscopically after the mice were sacrificed.ResultsThe difference of histological grading of kidney between the three groups was significant.The levels of BUN,Cr,MDA,SOD and GSH-Px between the GM group and GM+MLT group were significantly different(t=-10.41-9.55,P<0.01).ConclusionMelatonin provides certain protection against gentamicin-induced acute renal injury.The antioxidative functions of melatonin might be involved in the protective effects.