Dkk-1及Bcl-2蛋白在人鼻腔鼻窦鳞状细胞癌中的表达及意义

目的 探讨Dickkopf-1(DKK-1)及B细胞淋巴瘤/白血病-2 (B cell lymphoma/lewkmia-2 ,Bcl-2)蛋白在人鼻腔、鼻窦鳞状细胞癌(SNSCC)中的表达及其临床意义.方法 采用免疫组化SP法及Western blot技术分别检测30例SNSCC(SNSCC组) ,38例鼻腔、鼻窦内翻性乳头状瘤(SNIP组)以及20例中鼻甲黏膜组织(对照组)中DKK-1和Bcl-2蛋白的表达情况.结果与SNIP组及对照组比较 ,DKK-1蛋白在SNSCC组中的表达明显下调 ,Bcl-2蛋白在SNSCC组中的表达明显上调 ;在SNSCC组中 ,DKK-1及Bcl-2蛋白在高、中分化与低分化组中的阳性表达率分别为 100 .00% 、68 .75% 、33 .33% 及 50 .00% 、62 .50% 、100 .00% ,均差异有统计学意义(P＜0 .05).结论 DKK-1蛋白可能在SNSCC的发生和发展过程中起着重要的促进作用 ,且与Bcl-2蛋白呈负相关 ,DKK-1蛋白可能成为SNSCC基因治疗的新靶点.

Expression and significance of Dkk-1 and Bcl-2 protein in sinonasal squamous cell carcinoma

Objective To explore the expression and significance of Dickkopf-1 (Dkk-1) and cell lymphoma/leukemia-2 (Bcl-2) protein in sinonasal squamoucell carcinoma(SNSCC) .MethodThe immunohistochemical SP method and Western blomethod were adopted to determine the expression of Dkk-1 and Bcl-2 in 30 specimenof SNSC(SNSCgroup) ,38 specimenof sinonasal inverted papillomas(SNIP group) and 20 specimenof middle turbinate mucosa(control group) .ResultThe expression of DKK-1 protein in the SNSCgroup wasignificantly down-regulated compared with the SNIP group and the control group ,while the expression of Bcl-2 protein in the SNSCgroup wasignificantly up-regulated compared with the SNIP group and the control group;in the SNSCgroup ,the positive rateof DKK-1 protein and Bcl-2 protein in the high and middle differentiation group and the low differentiation group were 100 .00% ,68 .75% ,33 .33% and 50 .00% ,62 .50% ,100 .00% respectively ,the differencewere statistically significan(P＜0 .05) .Conclusion Dkk-1 protein may play an importanpromoting role in the developmenand pro-gression procesof SNSC,the expression of Dkk-1 protein hanegative correlation with the expression of Bcl-2 protein in SNSC,which may become new targespoof SNSCgene therapy .