用改良Hodge试验检测产碳青霉烯酶肠杆菌科细菌

目的了解分离的肠杆菌科细菌产碳青霉烯酶情况,为临床正确选择抗菌药物提供依据。方法采用K-B纸片扩散法检测并筛选厄他培南和美罗培南,以抑菌环直径分别为19~21 mm和16~21 mm的菌株作为待测菌,进行改良的Hodge试验。结果 390株肠杆菌科细菌中筛选出55株可疑产碳青霉烯酶菌株,对可疑菌株进行改良的Hodge试验,阳性率21.82%(12/55)。结论碳青霉烯类抗生素敏感折点附近的肠杆菌科细菌有近1/5菌株产碳青霉烯酶,临床微生物学实验室应执行改良Hodge试验,以确保药敏试验结果的准确性。

Detection of carbapenemase in isolates of Enterobacteriaceae by the modified Hodge test

Objective To investigate carbapenemase production in isolates of Enterobacteriaceae isolated from our hospital and provide a correct choice of antibiotic for the clinician.Methods Antibiotic susceptibility test was performed by K-B method.Strains showing zones of inhibition 19-21 mm with Ertapenem or 16-21 mm with Meropenem were screened as suspicious carbapenemase producing ones and tested for carbapenemase by the modified Hodge test.Results 55 of 390 strains of Enterobacteriaceae were suspected of possess...