| 人白介素24腺病毒载体的构建与鉴定 |
| |
| 引用本文： | 叶震敏,张颂文,缪竞诚,盛伟华,王伟录,吴晓阳,杨吉成. 人白介素24腺病毒载体的构建与鉴定[J]. 苏州大学学报(自然科学版), 2006, 26(1): 44-47 |
| |
| 作者姓名： | 叶震敏 张颂文 缪竞诚 盛伟华 王伟录 吴晓阳 杨吉成 |
| |
| 作者单位： | [1]苏州大学医学院细胞与分子生物学教研室,江苏苏州215123 [2]昆山市第一人民医院普外科,江苏昆山215300 |
| |
| 基金项目： | 苏州大学校科研和教改项目;江苏省昆山市科技局社会发展科技基金 |
| |
| 摘 要： | 目的构建人白介素24（hIL-24）的腺病毒载体，获得hIL-24重组腺病毒子，为hIL-24进行肿瘤的基因治疗奠定基础。方法以pcDNA3．0-hIL-24重组质料为模板，PCR扩增hIL-24，酶切连接到带有GFP标记的pAdTrack—CMVR质粒上，PmeI线性化重组质粒pAdTrack—CMV-hIL-24，与腺病毒质粒pAdEasy-1共转化BJ5183细菌，获得重组腺病毒载体pAdEasy-1-pAdTrack-CMV-hIL-24，经Pacl线性化后转染QBI-293A包装细胞。收获腺病毒重组病毒子，RT-PCR和Western—blotting鉴定。结果测序结果显示hIL-24序列正确，RT—PCR和Western-blotting检测到了hIL-24的表达。结论成功构建人白介素24的重组腺病毒载体pAdEasy-1-pAdTrack，CMV—hIL-24。获得了人白介素24重组病毒子Ad-hIL-24。
|
| 关 键 词： | 人白介素24 腺病毒载体 构建 鉴定 |
| 文章编号： | 1673-0399(2006)01-0044-04 |
| 收稿时间： | 2005-05-12 |
| 修稿时间： | 2005-05-12 |
The Construction and Identification of Recombinant Adenovirus Vector of hIL-24 |
| |
| YE Zhen-min, ZHANG Song-wen, MIAO Jing-cheng, et al. The Construction and Identification of Recombinant Adenovirus Vector of hIL-24[J]. Suzhou University Journal of Medical Science, 2006, 26(1): 44-47 |
| |
| Authors: | YE Zhen-min ZHANG Song-wen MIAO Jing-cheng et al |
| |
| Affiliation: | 1. Dept of Celluar and Molecular Biology, Medical School, Suzhou University, Jiangsu Suzhou 215123, China; 2. Dept of General Surgery, the First People Hospital of Kunshan, Jiangsu Kunshan 215300, China |
| |
| Abstract: | Objective The recombinant adenovirus vector of hIL-24 was constructed for carcinoma gene therapy.Methods The pAdTrack-CMV-hIL-24 was constructed by PCR with pcDNA3.0-hIL-24 recombinant plasmid as template,enzyme digestion and ligation.The pAdTrack-CMV-hIL-24(lineared) by PmeI was co-transformed into BJ5183 with pAdEasy-1.The pAdEasy-1-pAdTrack-CMV-hIL-24 recombinant adenovirus vector was lineared with PacI and then transfected into the package cell OBI-293A.The hIL-24 recombinant adenovirus was obtained and identified by RT-PCR and(Western-blotting.) Results The hIL-24 was confirmed by sequencing,and the expression of hIL-24 was detected by RT-PCR and Western-blotting.Conclusion The pAdEasy-1-pAdTrack-CMV-hIL-24 vector is constructed and the hIL-24 recombinant adenovirus(Ad-hIL-24)is obtained success fully. |
| |
| Keywords: | hIL-24 adenovirus vector construction identificaton |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
