Expression of CAPON after Spinal Cord Injury in Rats |
| |
Authors: | Chun Cheng Xin Li Shangfeng Gao Shuqiong Niu Mengling Chen Jing Qin Zhiqin Guo Jian Zhao Aiguo Shen |
| |
Institution: | (1) The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-xiu Road, Nantong, Jiangsu, 226001, China;(2) Department of Microbiology and Immunology, Nantong University (Former Nantong Medical College), Nantong, 226001, China;(3) Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, China |
| |
Abstract: | The adaptor protein, carboxy-terminal PDZ ligand of nNOS (CAPON), regulates the distribution of neuronal nitric oxide synthase
(nNOS) that increased after spinal cord injury (SCI) and produces the key signaling molecule nitric oxide (NO). But little
is known about the role of CAPON in the pathological process of SCI. The main objective of the present study was to investigate
expression of CAPON and nNOS in a spinal cord contusion model in adult rats. Real time-polymerase chain reaction (PCR) and
Western blot analysis revealed that mRNA and protein for CAPON increased at 2 h after SCI and reached the peak at 8 h, gradually
recovered to the baseline level at 14 days. The expression of nNOS mRNA and protein was similar to that of CAPON. During the
peak expression, CAPON mRNA was found in the ventral horn, mediate zone, dorsal horn, and white matter by in situ hybridization.
Immunofluorescence showed that CAPON was colocalized with nNOS in neurons, oligodendrocytes, and some astrocytes of spinal
cord tissues within 5 mm from the epicenter. Interaction between CAPON and nNOS was also detected by co-immunoprecipitation.
Thus, the transient expression of high levels of CAPON may provide new insight into the secondary response after SCI.
Chun Cheng and Xin Li contributed equally to this work. |
| |
Keywords: | Spinal cord injury CAPON nNOS Neuron Oligodendrocyte Astrocyte Rat |
本文献已被 PubMed SpringerLink 等数据库收录! |
|