雷帕霉素对人神经母细胞瘤细胞株生长抑制及诱导凋亡的研究

目的 研究雷帕霉素对人神经母细胞瘤细胞株SH-SY5Y生长抑制及诱导凋亡的作用.方法 雷帕霉素处理SH-SY5Y细胞,并设立对照组,以CCK-8检测细胞生长活性,流式细胞法(FCM)检测细胞周期,DNA梯度条带(DNA Ladder)和罗丹明123-碘化丙啶(Rhodamine 123/PI)染色法分析细胞凋亡.结果 雷帕霉素处理后,神经母细胞瘤细胞呈浓度依赖性生长抑制.20μM雷帕霉素处理12 h后,细胞周期出现G2/M期、S期阻滞,G2/M期(24.22±2.89)%、S期(22.05±0.96)%的细胞百分比明显高于对照组.罗丹明123-碘化丙啶检测细胞凋亡率(54.35±5.39)%也明显高于对照组,并有明显的凋亡条带出现.结论 雷帕霉素具有抑制神经母细胞瘤细胞株SH-SY5Y生长、诱导其凋亡的作用.

Rapamycin inhibits tumor growth and induces apoptosis in human neuroblastoma cells

Objective To study the effects of rapamycin on tumor growth and apoptosis in human neuroblastoma cells.Methods SH-SY5Y cells,human neuroblastoma cell line,were treated with rapamycin.Cell Counting Kit-8 (CCK-8)assay was employed to measure cell viability.Cell cycle was assessed using flow cytometry.Apoptosis was quantified by DNA Ladder and Rhodamine 123/PI staining.Results The concentration-dependent growth inhibition of SH-SY5Y cells were observed in rapamycin treatment groups.Cell cycle progression was arrested at G2/M phase and S phase after being treated with 20 μM rapamycin for 12 h.The percentage of cells at G2/M phase and S phase was 24.22± 2.89% and 22.05± 0.96%,respectively;which was significantly higher than those of the control groups.Furthermore,12 hours' treatment with 20 μM rapamycin resulted in clear apoptotic DNA ladder detection.The tumor cells' apoptosis was significantly higher after rapamycin treatment.Conclusions Rapamycin effectively inhibits tumor cell growth and induces apoptosis in human neuroblastoma cells.