多发性硬化病人血清和脑脊液壳三糖苷酶活性的研究

目的探讨多发性硬化(MS)病人血清和脑脊液(CSF)壳三糖苷酶(CTTS)活性以及CSF免疫活化和炎症标志物。方法选择三所医院178例MS病人,其中复发缓解型MS(RRMS)120例,继发进展型MS(SPMS)32例,原发进展型MS(PPMS)26例,并选取40例其他神经疾患(OND)和30非神经疾患病人作为对照组,检测血清和CSF中CTTS活性及CSF单核细胞数(MNC)和鞘内IgG产物。结果 MS病人与OND组和对照组比较,CSF中CTTS活性明显升高,但血清不升高。RRMS和SPMS组CTTS指数高于对照组,但PPMS组正常。在伴有MNC升高或CSF寡克隆IgG区带的MS病人,CTTS指数高于无此表现者。结论 RRMS和SPMS病人CCTS指数升高,CCTS指数与CSF炎症或免疫活化标志物有关。

Study of chitotriosidase activity in serum and cerebrospinal fluid in multiple sclerosis patients

Objective To investigate chitotriosidase(CTTS) activity in serum and cerebrospinal fluid(CSF) in multiple sclerosis(MS) patients and CSF markers for immune activation and inflammation.Methods We selected 178 patients from three hospitals,among them 120 patients were relapsing-remitting MS(RRMS),32 with secondary progressive MS(SPMS) and 26 with primary progressive MS(PPMS).While we chose 40 patients with other neurological disorders(OND) and 30 non-nervous system diseases patients as control group.We measured CTTS activity in serum and CSF and studied CSF mononuclear cell count(MNC) and intrathecal IgG production.Results CTTS activity was significantly higher in CSF in MS patients compared with OND and controls,but not in serum.In RRMS and SPMS,CTTS index was higher than controls,but not in PPMS.CCTS index was higher in MS patients with elevated MNC or CSF oligoclonal IgG bands than MS without these CSF findings.Conclusion CTTS elevated in RRMS and SPMS.CTTS index is related to CSF markers of immune activation or inflammation.