丙泊酚联合刺五加注射液对睡眠剥夺模型大鼠的药效学研究

目的 探讨丙泊酚联合刺五加注射液对睡眠剥夺模型大鼠的睡眠影响及机制.方法 睡眠剥夺模型SD大鼠随机分组为四组：空白对照组、丙泊酚组、刺五加组、丙泊酚-刺五加组.采用中缝背核（DRN）核团微量注射、多道睡眠描记、免疫组织化学技术和胆碱能受体放射性配基分析技术,观察大鼠在使用丙泊酚及刺五加注射液后睡眠生理行为、中缝核5-羟色胺（5-HT）能阳性神经元细胞数目,以及皮质神经细胞的胆碱能受体位点数变化.结果 丙泊酚组、刺五加组、丙泊酚-刺五加组的慢波睡眠（SWS）各期睡眠时间显著延长,联合应用后延长效果更显著,而觉醒期（w期）时间明显缩短（P〈0.05）;各组与对照组比较,大鼠大脑皮层5-HT阳性染色细胞明显增多,平均积分光密度值升高（P〈0.05）,联合应用后效果更显著.且多重比较显示,丙泊酚组和刺五加组问差异无显著性（P〉0.05）,其余各组间差异均存在显著性（P值均〈0.05）.各组间大鼠海马和皮层胆碱能受体位点数比较差异无统计学意义（P值分别为0.081、0.065、0.071）.结论 应朋丙泊酚、刺五加、丙泊酚联合刺五加背侧中缝核团微量注射可改善睡眠剥夺大鼠的睡眠.具体机制可能为通过增加大脑皮质细胞内5-HT能阳性物质发挥作用.海马和皮层胆碱能受体位点数改变可能不参与其作用机制.

Effects of propofol combined with ciwujia injection on the rat model of sleep deprivation

Objective To explore the effects of propofol combined with ciwujia injection on rats with sleep deprivation.Methods SD rats with sleep deprivation were randomly divided into placebo group,propofol group,ciwujia group,and propofol/ciwujia group.Microinjection into DRN,polysomnography (PSG),immunohistochemistrary,and the binding site (Bmax) and the affinity (KD) in the chonlinergic receptors were used in the experiment.The changes of sleep-awaking cycle,5-HT positive neurons,and cholinergic receptors of the cortical neural cells were observed in all the rats.Results The sleeping time of SWS elongated and phase W shortened noticeably in propofol group,ciwujia group,and propofol/ciwujia group (P<0.05).The number of 5-HT positive neurons increased,and no significant change in the number of cholinergic receptors of the,cortical neural cells were found in the latter three groups (P= 0.081,0.065,and 0.071; respectively) Conclusions Combination therapy with propofol and ciwujia and propofol can improve sleep in rats with sleep deprivation,whose mechanism may be the increase in 5-HT positive neurons of the cortical neural cells.