| 高效液相色谱法测定感冒止咳颗粒中黄芩苷含量 |
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| 引用本文： | 赵士冶,冯秀珍.高效液相色谱法测定感冒止咳颗粒中黄芩苷含量[J].中国现代药物应用,2009,3(12):11-12. |
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| 作者姓名： | 赵士冶 冯秀珍 |
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| 作者单位： | 湖南省常德市药品检验所,415000 |
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| 摘 要： | 目的建立感冒止咳颗粒中黄芩苷含量测定方法。方法采用Uhimate TM XB-C18色谱柱（4．6mm×200mm，5μm）；流动相：甲醇-0．4％磷酸（50：50）；流速：1．0ml/min；检测波长：277nm；柱温：40％。结果黄芩苷的线性范围为0．058～1．16μg（R：=1．000），平均回收率为99．54％，RSD=0．85％（n=6）。结论该方法准确可靠、简单快速，可用于感冒止咳颗粒质量控制。
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| 关 键 词： | 高效液相色谱法 感冒止咳颗粒 黄芩苷 |
Determination of baicalin in Ganmaozhike granules by HPLC |
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| ZHAO Shi-ye,FENG Xiu-zhen.Determination of baicalin in Ganmaozhike granules by HPLC[J].Chinese Journal of Modern Drug Application,2009,3(12):11-12. |
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| Authors: | ZHAO Shi-ye FENG Xiu-zhen |
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| Institution: | . (Hunan Province Changde Institute for Drug Control, Changde, Hunan 415000, China) |
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| Abstract: | Objective To establish an HPLC method for content determination of Ganmaozhike gran- ules. Methods The separation was performed with an UltimateTM XB-C18 column （4. 6 mm × 200 mm, 5 μm）and the mobile phase was methol-0.4% phosphoric acid（50 ： 50）. The flow rate was 1.0 mL/min and the wavelength of detection was 277 nm. The column temperature was 40℃. Results The liner range of baicalin was 0. 058 - 1.16 μg（ R2 = 1. 000） and the average recovery was 99. 54%, RSD = 0. 85% （ n = 6 ）. Conclusion This method is accurate,simple and can be used for the quality control of Ganmaozhike granules. |
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| Keywords: | HPLC Ganmaozhike granules Baicalin |
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